Anti-CDC5L Rabbit Polyclonal Antibody for WB, ELISA - Q99459

Core Product Specifications and Parameters

Biological Background: CDC5L Function and Localization

• Protein Identity: CDC5L (Cell division cycle 5-like protein), also known as pombe cdc5-related protein (PCDC5RP), is a DNA-binding protein encoded by the CDC5L gene (Gene ID: 988).
• Core Function in Splicing: It is a core component of precatalytic, catalytic, and postcatalytic spliceosomal complexes, playing a critical role in pre-mRNA splicing. Related references: PMID:11991638 PMID:20176811 PMID:28076346
• PRP19-CDC5L Complex: It is an integral component of the PRP19-CDC5L complex, which is required for activating pre-mRNA splicing and may also participate in the DNA damage response (DDR). Related references: PMID:20176811
• Minor Spliceosome Involvement: As a component of the minor spliceosome, CDC5L is involved in the splicing of U12-type introns in pre-mRNAs (Probable).
• Transcriptional Regulation: It may act as a transcription activator, suggesting a dual role in gene expression control.
• Subcellular Localization: CDC5L localizes predominantly to the nucleus and nuclear speckles, with some cytoplasmic presence, consistent with its roles in splicing and transport.
• Tissue Distribution: It is ubiquitously expressed in both fetal and adult tissues, underscoring its fundamental cellular functions.
• Post-Translational Modifications: The protein is subject to phosphorylation, isopeptide bond formation (ubiquitination), and other modifications, as indicated by keywords: Phosphoprotein, Isopeptide bond, Ubl conjugation.

Experimental Guidance and Technical Tips

• Immunogen Design: The antibody was raised against a recombinant protein covering amino acids 563-802, a region near the C-terminus. When considering alternative immunogens, focus on this domain to maintain specificity.
• Western Blot (WB): Predicted molecular weight is 92 kDa; full-length endogenous CDC5L may migrate around this size. Validate detection in relevant human or mouse lysates.
• ELISA Application: The antibody is qualified for ELISA; consider indirect ELISA with the immunogen as coating antigen for titer determination.
• Cross-Reactivity: Detects both human and mouse CDC5L due to sequence conservation. Verify reactivity in other species if needed.
• Sample Preparation: As a nuclear protein, consider using nuclear extracts to enrich the signal in WB.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop IVD raw-material sourcing partner, bridging the gap from concept to clinic. For CDC5L-targeted assay development, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies like this rabbit pAb, and bulk ancillary reagents. Our platform also supports rare target sourcing and technical consultation, accelerating your diagnostic projects.