Anti-CDC45 Polyclonal Antibody for WB, ELISA - O75419

Core Product Specifications and Parameters

Biological Background: CDC45 Function and Localization

• CDC45, also known as CDC45L or PORC-PI-1, is a cell division control protein essential for the initiation of chromosomal DNA replication.
• It is a core component of the CDC45-MCM-GINS (CMG) helicase complex, which unwinds double-stranded DNA at replication forks and serves as the scaffold for replisome assembly.
• The protein localizes predominantly to the nucleus and is chromosome-associated, consistent with its function in DNA replication.
• CDC45 is widely expressed in human tissues, with the highest mRNA levels observed in adult testis, thymus, and fetal liver.
• Post-translational modifications include phosphorylation (Phosphoprotein keyword), and the protein is subject to alternative splicing, producing multiple isoforms.
• Mutations in CDC45 have been linked to disease, including Meier-Gorlin syndrome (dwarfism), highlighting its importance in cell cycle regulation and genomic integrity.
• The calculated molecular mass is approximately 66 kDa (UniProt: 65.6 kDa), and its three-dimensional structure has been determined, providing insights into helicase activation.

Experimental Guidance and Technical Tips

• The immunogen comprises the N-terminal 1–270 amino acids of human CDC45, a region expected to generate antibodies recognizing full-length endogenous protein.
• For Western blotting, a specific band around 66 kDa is expected; using nuclear-enriched lysates or synchronizing cells in S-phase may enhance signal.
• Validate antibody performance in your model system with appropriate controls, such as siRNA-mediated knockdown or genetic knockout, to confirm specificity.
• Cross-reactivity with mouse and rat has been reported; however, validation for each species is recommended due to potential differences in sequence homology.
• For ELISA, the polyclonal nature can provide robust signal; consider testing the antibody as a capture reagent paired with a monoclonal detection antibody for sandwich ELISA development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner, offering a complete portfolio from R&D-grade antibodies to bulk production lots. This anti-CDC45 polyclonal antibody is part of our expanding catalog supporting DNA replication and cell cycle research. We also provide customized antibody development, antigen production, and ancillary reagent sourcing to streamline your assay development pipeline.