Anti-CDC42EP1 Monoclonal Antibody for WB, IHC-P, ELISA - Q00587

Core Product Specifications and Parameters

Biological Background: CDC42EP1 Function and Localization

• CDC42EP1, also known as Binder of Rho GTPases 5 (BORG5) or Serum protein MSE55, is a Cdc42 effector protein encoded by the CDC42EP1 gene (GeneID: 11135).
• It is involved in the organization of the actin cytoskeleton and has been shown to induce membrane extensions in fibroblasts.
• The protein localizes to the endomembrane system, cytoplasm, and cytoskeleton.
• It is specifically expressed in endothelial and bone marrow stromal cells.
• Post-translational modifications include phosphorylation and methylation, as identified by proteomics.
• Alternatively spliced isoforms exist, contributing to functional diversity.
• The protein contains repeat regions and has a calculated molecular weight of approximately 40 kDa (UniProt: 40295 Da).

Experimental Guidance and Technical Tips

• The immunogen was designed against amino acids 292–391; for Western blotting, expect a band near 40 kDa. Confirm specificity by using positive and negative controls.
• For IHC-P, optimize antigen retrieval and validate staining patterns in endothelial-rich tissues or bone marrow stroma.
• Cross-reactivity with mouse and rat is reported; consider sequence alignment to predict reactivity, and validate in relevant animal tissue samples.
• As a monoclonal antibody, it provides consistent target recognition; titrate to determine the optimal working dilution for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs as a one-stop sourcing partner for IVD raw materials. In addition to validated monoclonal antibodies like this anti-CDC42EP1 clone, we provide optimized antibody pairs, bulk ancillary reagents, and rare target raw-material procurement services. Our infrastructure helps accelerate research on actin cytoskeleton dynamics and endothelial cell biology from concept to clinic.