Anti-CDC42 Rabbit Polyclonal Antibody for IF/ICC, ELISA - P60953

Core Product Specifications and Parameters

Biological Background: CDC42 Function and Localization

• CDC42 is a plasma membrane-associated small GTPase that cycles between an active GTP-bound state and an inactive GDP-bound state, regulating various cellular responses.
• It plays a critical role in epithelial cell polarization, bipolar spindle microtubule attachment to kinetochores, and cell migration.
  Related references:
  PMID:15642749
  PMID:17038317
  PMID:22843693
• In neurons, CDC42 is involved in the extension and maintenance of filopodia and is required for DOCK10-mediated spine formation in Purkinje cells and hippocampal neurons.
  Related references:
  PMID:14978216
• It participates in phagocytosis by organizing the F-actin cytoskeleton around phagocytic cups and mediates actin cytoskeletal remodeling during epithelial cell-cell junction formation upon activation by PLEKHG4B.
  Related references:
  PMID:26465210
  PMID:33310911
• The protein localizes to the cell membrane, cytoplasm, centrosomes, spindles, midbody, dendritic projections, and secretory vesicles such as acrosomes.
• Post-translational modifications include phosphorylation, prenylation, methylation, and ubiquitination, which regulate its subcellular targeting and activity.
• Alternative splicing generates multiple isoforms, and mutations in CDC42 are associated with intellectual disability.
• The predicted molecular weight is approximately 21.3 kDa (observed at ~21 kDa in SDS-PAGE).

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was raised against a recombinant protein corresponding to amino acids 1-186 of human CDC42 (isoform 1). It is expected to recognize endogenous CDC42 in human samples.
• For immunofluorescence/immunocytochemistry (IF/ICC), a typical starting dilution might be considered; however, optimal working concentration should be empirically determined using appropriate positive and negative controls.
• For ELISA, the antibody can be used for antigen capture or detection; pairing with a matched detection antibody may improve sensitivity.
• Given the antibody's polyclonal nature, lot-to-lot variability may occur; validation in the relevant biological system is advisable.
• The predicted band size in Western blot is ~21 kDa, although this application is not listed; cross-reactivity with other Rho family GTPases cannot be excluded.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and research communities by providing a comprehensive range of IVD raw materials and technical services, from antibody discovery to manufacturing. This anti-CDC42 polyclonal antibody addresses needs in cytoskeletal dynamics and cell migration research, and we can further assist with custom antibody development, bulk reagent supply, and sourcing of rare targets. Our platform streamlines procurement, ensuring timely access to validated tools for your diagnostic and laboratory workflows.