Anti-CDC37 Rabbit Polyclonal Antibody for WB, IP, ELISA - Q16543

Core Product Specifications and Parameters

Biological Background: CDC37 Function and Localization

• CDC37, also known as p50Cdc37, is a 44468 Da Hsp90 co-chaperone that targets protein kinases (UniProt: Q16543).
• It binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and enhancement of their activity (PubMed:8666233). Related references: PMID:8666233
• CDC37 inhibits the ATPase activity of Hsp90 (HSP90AA1) (PubMed:23569206). Related references: PMID:23569206
• Subcellular localization is primarily cytoplasmic, consistent with its role in chaperone complexes.
• Post-translational modifications include phosphorylation, acetylation, and ubiquitination (Ubl conjugation), which may regulate its function.
• Keywords: Chaperone, Cytoplasm, Phosphoprotein, Acetylation, Ubl conjugation, Reference proteome; structure available (3D-structure).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1-160 amino acids of human CDC37, which is highly conserved; cross-reactivity with mouse has been reported, but users should verify in their experimental system.
• For Western blotting, a band at approximately 44 kDa is expected. Consider using appropriate positive controls and optimizing antibody dilution.
• In immunoprecipitation, confirm target enrichment by Western blot with the same or a different antibody.
• For ELISA, the antibody can be used as a capture or detection reagent; validate in the relevant sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-CDC37 rabbit polyclonal antibody supports research in kinase signaling and chaperone biology. CamelBio also offers technical consulting and rare target sourcing to accelerate your assay development from concept to clinic.