Anti-CDC37 Rabbit Monoclonal Antibody for WB and ELISA - Q16543

Core Product Specifications and Parameters

Biological Background: CDC37 Function and Localization

• CDC37 encodes the Hsp90 co-chaperone Cdc37 (also known as p50Cdc37), a key regulator of protein kinase maturation.
• Binds to numerous protein kinases and promotes their interaction with the Hsp90 chaperone complex, thereby stabilizing the kinases and enhancing their activity.
  Related references: PMID:8666233
• Inhibits the ATPase activity of HSP90AA1, fine-tuning the chaperone cycle.
  Related references: PMID:23569206
• Predominantly cytoplasmic, consistent with its role in chaperone-mediated kinase processing.
• Subject to multiple post-translational modifications, including phosphorylation and acetylation, which may regulate its co-chaperone function.
• Structural studies (3D-structure annotation in UniProt) have elucidated its interaction interfaces with Hsp90 and client kinases.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering the N‑terminus (amino acids 1–100) of human CDC37; the resulting antibody is expected to recognize the linear epitope in denaturing Western blot conditions.
• Validate the antibody in relevant sample systems expressing CDC37 (e.g., HeLa, HEK293) and confirm band at ~44 kDa.
• For ELISA, consider optimizing coating antigen concentration and detection conditions; the antibody is suitable for both direct and sandwich ELISA formats.
• Cross‑reactivity with mouse and rat CDC37 has been supplier‑verified; in silico sequence alignment may further support use in these species.

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CamelBio is a dedicated one‑stop sourcing platform for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. For researchers and diagnostic developers working on kinase signaling and chaperone biology, CamelBio streamlines procurement of reagents like this anti‑CDC37 rabbit monoclonal antibody, together with technical and supply‑chain support to accelerate development from concept to clinic.