Anti-CDC34 Rabbit Monoclonal Antibody for WB, ELISA - P49427

Core Product Specifications and Parameters

Biological Background: CDC34 Function and Localization

• CDC34 (UBE2R1) is an E2 ubiquitin-conjugating enzyme that accepts ubiquitin from E1 and catalyzes its covalent attachment to substrate proteins with E3 ligase complexes (PubMed:10329681, PubMed:17588522, PubMed:20061386). In vitro, it catalyzes Lys-48-linked polyubiquitination (PubMed:22496338). Related references: PMID:10329681, PMID:17588522, PMID:20061386, PMID:22496338
• It cooperates with UBCH5C and the SCF(FBXW11) E3 ligase for polyubiquitination and proteasomal degradation of NFKBIA (IκBα), playing a role in NF-κB signaling (PubMed:10329681, PubMed:10918611, PubMed:17698585). Related references: PMID:10329681, PMID:10918611, PMID:17698585
• Involved in cell cycle regulation by targeting cell cycle regulators for ubiquitination and degradation: cooperates with SCF(SKP2) to degrade MYBL2 and KIP1 (p27) (PubMed:10871850, PubMed:15652359, PubMed:19112177), and mediates WEE1 degradation to control G2/M transition (PubMed:19126550). Related references: PMID:10871850, PMID:15652359, PMID:19112177, PMID:19126550
• Also contributes to the degradation of beta-catenin (PubMed:12037680) and the transcription repressors ICERIIgamma and ATF5, relieving cAMP-induced transcriptional repression during spermatogenesis. Related references: PMID:12037680
• Subcellular localization is both cytoplasmic and nuclear, consistent with its diverse substrate range.
• Expressed in testes during spermatogenesis to regulate repression of cAMP-induced transcription.
• Post-translational modifications include phosphorylation; also known to interact with viral protein ICP0 from human herpes virus 1.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 20-236 of human CDC34, covering the core UBC domain; thus the antibody is expected to detect both unmodified and modified forms. For western blot, expect a band at ~27 kDa in human cell lysates; validation with knockdown or overexpression models is recommended.
• Suitable for ELISA; consider using recombinant CDC34 protein as a positive control and performing titration to determine optimal coating and antibody concentrations.
• Due to high conservation, cross-reactivity with other species is not guaranteed; preliminary testing in the target species is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing platform for IVD raw materials, technical services, and consulting—from concept to clinic. This anti-CDC34 monoclonal antibody is offered to support research on ubiquitin-mediated proteolysis and cell cycle control, with additional supply capabilities including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing of rare target raw materials.