Anti-CDC25C Rabbit Polyclonal Antibody for WB, ELISA - P30307

Core Product Specifications and Parameters

Biological Background: CDC25C Function and Localization

• CDC25C (M-phase inducer phosphatase 3) is a dual specificity phosphatase that plays a critical role in cell cycle regulation, specifically at the G2/M transition.
• It functions as a dosage-dependent inducer in mitotic control; when phosphorylated, it potently drives G2 cells into prophase by directly dephosphorylating and activating CDK1 kinase. Related references: PMID:8119945
• The protein is localized predominantly in the nucleus, consistent with its role in regulating nuclear events of mitosis.
• CDC25C is subject to regulatory phosphorylation, and its activity is finely tuned during the cell cycle; it is also acetylated and undergoes alternative splicing.
• As a member of the protein phosphatase family, it belongs to the broader class of hydrolases and is implicated in host-virus interactions, though detailed mechanisms remain to be fully elucidated.
• The canonical isoform has a predicted molecular weight of approximately 53 kDa.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 20–130 of human CDC25C, a region that may be conserved across species; thus, the antibody is expected to cross-react with mouse and rat CDC25C based on supplier data. However, sequence alignment should be checked for your specific model.
• For Western blotting (WB), a band corresponding to the ~53 kDa protein is anticipated; however, phosphorylation events may cause slight mobility shifts. Consider using phosphatase inhibitors during sample preparation to preserve native modification states.
• In ELISA applications, the antibody can be used to detect recombinant or endogenous CDC25C; pairing with a suitable detection system and standard curve is recommended for quantification.
• As a polyclonal antibody, potential lot-to-lot variability exists; it is advisable to pre-validate each new batch in your assay system.
• Always include appropriate positive and negative controls (e.g., CDC25C-overexpressing lysates or knockdown samples) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target raw‑material sourcing. For cell cycle research and diagnostic development targeting CDC25C or related mitotic regulators, CamelBio streamlines procurement from concept to clinic.