Anti-Cdc25C Monoclonal Antibody for WB, IF/ICC, ELISA - P30307

Core Product Specifications and Parameters

Biological Background: Cdc25C Function and Localization

• Cdc25C, also known as M-phase inducer phosphatase 3 and dual specificity phosphatase Cdc25C, is a key tyrosine phosphatase controlling G2/M transition.
• It functions as a dosage-dependent inducer of mitotic progression by directly dephosphorylating and activating CDK1, which is essential for driving cells from G2 into prophase. Related references: PMID:8119945
• The protein is predominantly localized to the nucleus, where it regulates nuclear CDK1/cyclin B complexes.
• Alternative splicing generates multiple isoforms, potentially contributing to functional diversity.
• Post-translational modifications, including phosphorylation and acetylation, modulate Cdc25C activity and stability.
• UniProt keywords such as Cell cycle, Cell division, Mitosis, Nucleus, Phosphoprotein, and Host-virus interaction highlight its central role in cell division and possible involvement in viral processes.
• The calculated molecular weight of Cdc25C is approximately 53 kDa (53,365 Da).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning the N-terminal region (aa 1–100) of human Cdc25C, which may contain key regulatory phosphorylation sites; thus, the antibody might recognize both unphosphorylated and phosphorylated forms.
• For Western blot (WB), consider validating specificity with phosphatase-treated lysates or peptide competition assays to confirm band identity.
• In immunofluorescence/immunocytochemistry (IF/ICC), permeabilization is required for nuclear access; fixation conditions should be optimized to preserve epitope accessibility.
• For ELISA, this antibody can be used as a capture or detection reagent for recombinant or purified Cdc25C protein; perform standard titration and buffer optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including high-quality Cdc25C antibodies for cell cycle and oncology research applications. As a B2B sourcing partner, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom raw-material sourcing to support diagnostic development from concept to clinic.