Anti-CDC25B Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P30305

Core Product Specifications and Parameters

Biological Background: CDC25B Function and Localization

• Encodes a dual specificity phosphatase that functions as a dosage-dependent inducer of mitotic progression by directly dephosphorylating and activating CDK1. Related references: PMID:1836978 PMID:20360007
• Required for the G2/M transition of the cell cycle and participates in abscission during cytokinesis in an ECT2-dependent manner. Related references: PMID:17332740
• The three isoforms exhibit different levels of phosphatase activity, potentially contributing to differential regulation of cell division. Related references: PMID:1836978
• Localizes to the cytoplasm, centrosome, and spindle pole during mitosis, coordinating cell cycle progression at the microtubule organizing center.
• Belongs to the dual specificity protein phosphatase family and is regulated by alternative splicing, generating multiple isoforms.
• Functions as a phosphoprotein and hydrolase tightly controlled by phosphorylation and 14-3-3 binding, integrating signals for the G2/M checkpoint.

Experimental Guidance and Technical Tips

• The immunogen targets amino acids 100–200 of human CDC25B; consider using this peptide for blocking experiments to confirm antibody specificity.
• For Western blot, anticipate a band near 65 kDa. Use lysates from proliferating cells (e.g., HeLa) and validate with cell cycle synchronization to confirm expression.
• In IHC-P, antigen retrieval conditions and antibody dilution may require optimization; staining is expected in nuclear and cytoplasmic compartments, influenced by cell cycle phase.
• ELISA can be utilized for quantitative detection or binding assays; include appropriate negative and positive controls to ensure reliability.
• Cross-reactivity with other species has not been verified; validate in the relevant sample model if extending applications beyond human.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For your cell cycle and mitosis research, CamelBio supplies a comprehensive portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. We are dedicated to bridging your procurement needs with reliable supply solutions for CDC25B and related targets.