Anti-CDC25A Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P30304

Core Product Specifications and Parameters

Biological Background: CDC25A Function and Localization

• M-phase inducer phosphatase 1 (CDC25A) is a dual specificity phosphatase that acts as a dosage-dependent inducer of mitotic progression (PubMed:12676925, PubMed:14559997, PubMed:1836978). Related references: PMID:12676925, PMID:14559997, PMID:1836978
• It directly dephosphorylates CDK1 and stimulates its kinase activity; it also dephosphorylates CDK2 in complex with cyclin E in vitro (PubMed:20360007). Related references: PMID:20360007
• The CDC25A gene undergoes alternative splicing, generating multiple protein isoforms (UniProt keyword: Alternative splicing).
• The protein is classified under the keywords cell cycle, cell division, and mitosis, underscoring its central role in cell proliferation.
• Post-translational modifications include phosphorylation (Phosphoprotein keyword) and ubiquitination (Ubl conjugation), which regulate CDC25A stability and activity.
• CDC25A is a reference proteome component and has been identified in several proteomics studies (Proteomics identification keyword).

Experimental Guidance and Technical Tips

• The immunogen (recombinant protein corresponding to amino acids 86-328) covers a central region; consider validating antibody specificity in knockout models or with blocking peptides.
• For Western blot detection, expect a band around 59 kDa; note that phosphorylation may cause migration shifts. Use appropriate phosphatase inhibitors.
• In immunohistochemistry (IHC-P), optimize antigen retrieval conditions and validate staining in tissues with known CDC25A expression, such as proliferating tissues or cancer samples.
• For immunofluorescence, test multiple fixation methods to maintain antigenicity and subcellular localization.
• As a polyclonal antibody, it may recognize multiple CDC25A isoforms; confirm with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with comprehensive access to high-quality IVD raw materials like this anti-CDC25A antibody. We offer a full range of validated antibody pairs, optimized monoclonals/polyclonals, and ancillary reagents for cell cycle and oncology research. For rare target sourcing, our team ensures a reliable supply chain to support your assay development from concept to clinic.