Anti-CDC25A Rabbit Monoclonal Antibody for WB, ELISA - P30304

Core Product Specifications and Parameters

Biological Background: CDC25A Function and Localization

• CDC25A (M-phase inducer phosphatase 1, also known as Dual specificity phosphatase Cdc25A) is a key regulator of cell cycle progression.
• It functions as a dosage-dependent inducer of mitotic progression through its tyrosine phosphatase activity. Related references: PMID:12676925 PMID:14559997 PMID:1836978
• CDC25A directly dephosphorylates and activates CDK1, a master mitotic kinase. Related references: PMID:20360007
• It also dephosphorylates CDK2 in complex with cyclin E, contributing to G1/S transition regulation. Related references: PMID:20360007
• The protein undergoes alternative splicing, generating multiple isoforms with potentially distinct functions.
• CDC25A is a phosphoprotein and is regulated by ubiquitin conjugation (Ubl conjugation), which targets it for proteasomal degradation during DNA damage checkpoints.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight of CDC25A isoforms is 54-59 kDa; consider using positive control lysates from human, mouse, or rat cell lines and optimizing transfer conditions for clear band resolution.
• A range of primary antibody concentrations (e.g., 0.5-2 µg/mL) should be tested to determine the optimal signal-to-noise ratio in both WB and ELISA setups.
• Validate cross-reactivity in mouse and rat samples using appropriate tissue or cell extracts to confirm species-specific performance.
• For ELISA applications, pairing this antibody with a matched detection antibody may enhance sensitivity; run standard curves with target recombinant protein to assess assay linearity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including this anti-CDC25A rabbit monoclonal antibody, to support cell cycle and cancer research. As your sourcing partner, we supply validated antibody pairs, optimized recombinant proteins, and ancillary reagents—alongside tailored solutions for rare or difficult targets like cell cycle phosphatases. Simplify your supply chain with CamelBio’s integrated procurement from concept to clinic.