Anti-CDC23 Rabbit Monoclonal Antibody for WB, ELISA - Q9UJX2

Core Product Specifications and Parameters

Biological Background: CDC23 Function and Localization

• CDC23, also known as APC8 or ANAPC8, is a core subunit of the anaphase-promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase essential for cell cycle progression. Related references: PMID:18485873, PMID:27120157, PMID:27509861
• APC/C mediates ubiquitination and subsequent proteasomal degradation of key cell cycle regulators, primarily forming 'Lys-11'-linked polyubiquitin chains, and to a lesser extent 'Lys-48'- and 'Lys-63'-linked chains; it also catalyzes branched 'Lys-11'/'Lys-48'-linked ubiquitin chains. Related references: PMID:18485873, PMID:29033132
• The complex is activated by CDC20 during the metaphase-to-anaphase transition, targeting cyclin B and securin for degradation to trigger chromosome separation and mitotic exit. Related references: PMID:27120157, PMID:27509861
• APC/C activity is tightly controlled by the mitotic checkpoint complex (MCC), which inhibits APC/C to delay anaphase until all chromosomes are properly attached to the spindle. Related references: PMID:27509861
• The CDC23 protein contains TPR repeats, which are likely involved in protein-protein interactions within the APC/C complex.
• CDC23 is subject to post-translational modifications, including phosphorylation and acetylation, potentially regulating its activity or interactions during the cell cycle.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide (aa 498-597), suggesting the antibody likely recognizes a linear epitope, making it suitable for denaturing Western blotting.
• For Western blotting, expect a band near 69 kDa; validate specificity using appropriate positive and negative controls (e.g., knockdown or knockout lysates).
• For ELISA, consider titrating the antibody to determine optimal working concentrations for capture or detection, and validate in the relevant sample matrix.
• Cross-reactivity is reported for human, mouse, and rat; if working with other species, verify sequence homology of the immunogen region first.

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CamelBio is your dedicated partner for IVD raw-material sourcing, connecting diagnostic manufacturers and research laboratories with high-quality reagents like this anti-CDC23 monoclonal antibody for cell cycle and oncology research. Beyond individual antibodies, we supply validated antibody pairs, bulk ancillary reagents, and tailored support for sourcing rare targets, streamlining your journey from concept to clinic.