Anti-CDC20 Polyclonal Antibody for WB, IF/ICC, ELISA - Q12834

Core Product Specifications and Parameters

Biological Background: CDC20 Function and Localization

• CDC20 is a substrate-specific adapter and activator of the anaphase promoting complex/cyclosome (APC/C), targeting substrates for ubiquitination and degradation. Related references: PMID:9734353 PMID:27030811 PMID:29343641

• It is essential for the metaphase/anaphase transition by targeting cyclin B and securin for degradation. Related references: PMID:27120157 PMID:27509861 PMID:32666501

• Recognizes destruction box (D box), KEN box and ABBA motifs on substrates, guiding specific recognition. Related references: PMID:29343641 PMID:27509861

• Component of the mitotic checkpoint complex (MCC), which inhibits APC/C until chromosomes are properly attached to the mitotic spindle. Related references: PMID:27509861

• The MCC includes CDC20, MAD2L1, BUB1B, and BUB3; it delays the metaphase/anaphase transition. Related references: PMID:9811605 PMID:9637688 PMID:27509861

• When the spindle checkpoint is silenced, CDC20 is auto-ubiquitinated in the MCC, reactivating APC/C for anaphase. Related references: PMID:27509861

• Subcellularly, CDC20 localizes to the cytoplasm, centrosome, kinetochore, and spindle pole during mitosis.

• CDC20 contains WD repeats and is regulated by acetylation, phosphorylation, and ubiquitination; mutations are associated with disease.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 1–270) covers the N-terminal region, which may be involved in substrate recognition; the antibody could recognize both native and denatured forms.
• For Western blot, a band near 55 kDa is expected; consider using mitotic cell lysates to enrich for CDC20.
• In immunofluorescence, staining should be observed at mitotic structures such as kinetochores and spindle poles; validate in synchronized cells.
• Cross-reactivity with mouse allows for studies in mouse models; verify reactivity in your specific strain if needed.
• For ELISA, optimize coating conditions and antibody titration for your assay format.
• As a polyclonal antibody, lot-to-lot variability is possible; include appropriate controls and consider affinity purification if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to IVD raw materials for targets like CDC20, a key regulator of cell cycle progression and a biomarker in oncology research. Our portfolio includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and sourcing support for rare targets. Let CamelBio be your reliable partner from concept to clinic, ensuring quality and efficiency at every stage.