Anti-CDC16 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q13042

Core Product Specifications and Parameters

Biological Background: CDC16 Function and Localization

• CDC16 (APC6) is a core component of the anaphase-promoting complex/cyclosome (APC/C), an E3 ubiquitin ligase that targets key cell cycle regulators for proteasomal degradation PMID:18485873. Related references: PMID:18485873
• The APC/C primarily catalyzes the formation of Lys-11-linked polyubiquitin chains and can assemble branched Lys-11/Lys-48-linked chains on substrates PMID:18485873 PMID:29033132. Related references: PMID:18485873 PMID:29033132
• APC/C activation by CDC20 at the metaphase/anaphase transition triggers the degradation of cyclin B and securin to drive mitotic exit PMID:27120157 PMID:27509861. Related references: PMID:27120157 PMID:27509861
• The mitotic checkpoint complex (MCC) inhibits APC/C to delay chromosome segregation until all chromosomes are properly attached PMID:27509861. Related references: PMID:27509861
• CDC16 localizes to the cytoplasm, centrosome, and mitotic spindle, consistent with its role in cell division.
• The protein contains multiple TPR repeat motifs and is phosphorylated, likely regulating APC/C assembly and interactions with substrates and cofactors.
• Alternative splicing yields isoforms that may confer tissue-specific or context-dependent APC/C functions.

Experimental Guidance and Technical Tips

• The immunogen (residues 371–620) spans a region expected to be surface-exposed and involved in TPR-mediated protein interactions; consider this for designing orthogonal validation experiments.
• For Western blot, expect a band near 72 kDa; phosphorylated forms may exhibit slight shifts. Use appropriate protease and phosphatase inhibitors during lysis.
• In immunohistochemistry (IHC-P), optimize antigen retrieval and antibody dilution; validate staining patterns using known positive and negative control tissues.
• For ELISA applications, test as both capture and detection antibody against recombinant CDC16 or APC/C complexes; include appropriate positive and negative controls.
• Due to cross-reactivity with mouse and rat, confirm specificity in the target species using knockdown or knockout controls if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio accelerates IVD assay development by offering a reliable supply of high-quality antibodies and ancillary reagents for cell cycle and ubiquitination research. Our expertise in validated antibody pairs and recombinant proteins supports the building of robust detection systems targeting APC/C subunits like CDC16. Let CamelBio be your integrated partner for raw materials and technical support, from concept to clinical application.