Anti-CDC16 Rabbit Monoclonal Antibody for IHC-P, IF/ICC, ELISA - Q13042

Core Product Specifications and Parameters

Biological Background: CDC16 Function and Localization

• CDC16 (APC6) is a core subunit of the anaphase promoting complex/cyclosome (APC/C), a multi-subunit E3 ubiquitin ligase essential for cell cycle progression. Related references: PMID:18485873
• APC/C mediates ubiquitination and subsequent proteasomal degradation of key cell cycle regulators, primarily catalyzing 'Lys-11'-linked polyubiquitin chains, and to a lesser extent 'Lys-48'- and 'Lys-63'-linked chains. It can also assemble branched 'Lys-11'/'Lys-48' chains on substrates. Related references: PMID:18485873, PMID:29033132
• Activation of APC/C by CDC20 in metaphase triggers the degradation of securin and cyclin B, promoting the transition to anaphase and mitotic exit. Related references: PMID:27120157, PMID:27509861
• APC/C activity is inhibited by the mitotic checkpoint complex (MCC) to ensure accurate chromosome segregation until all kinetochores are properly attached. Related references: PMID:27509861
• CDC16 localizes to the cytoplasm, centrosomes, and mitotic spindle during cell division, consistent with its role in mitotic regulation.
• CDC16 belongs to a family of APC/C subunits characterized by tetratricopeptide repeat (TPR) domains that mediate protein-protein interactions.
• The protein undergoes phosphorylation, which may regulate its activity or interactions within the complex.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 200-300 of human CDC16. This internal sequence may be suitable for detecting full-length CDC16 in western blot applications, though not included in the standard application list, consider validating for denaturing protein analysis given the predicted molecular weight of ~72 kDa.
• For IHC-P, consider using heat-induced antigen retrieval (e.g., citrate buffer, pH 6.0) and optimise primary antibody concentration.
• In IF/ICC, ensure proper fixation (e.g., 4% paraformaldehyde) and permeabilization to allow access to cytoplasmic and centrosomal CDC16.
• ELISA may be used to quantify CDC16; titrate the antibody and validate cross-reactivity with your target species (human, mouse, rat).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For researchers studying cell cycle regulation and ubiquitin ligases, this CDC16 monoclonal antibody is supported by CamelBio's comprehensive portfolio, including validated antibody pairs, bulk ancillary reagents, and rare target sourcing. Our integrated supply chain ensures consistent quality and rapid delivery for your IVD development needs.