Anti-CDA Rabbit Monoclonal Antibody for IHC-P, FC (intra), ELISA - P32320

Core Product Specifications and Parameters

Biological Background: CDA Function and Localization

• CDA encodes cytidine deaminase (cytidine aminohydrolase), a zinc-dependent enzyme belonging to the hydrolase family.
• The enzyme catalyzes the hydrolytic deamination of cytidine and 2'-deoxycytidine to uridine and 2'-deoxyuridine, respectively, playing a key role in the pyrimidine salvage pathway for UMP synthesis.
• CDA is highly expressed in granulocytes, with very low expression in fibroblasts, chondrocytes, monocytes, and T- and B-cell lines.
• The protein requires zinc as a cofactor essential for its catalytic activity and metal-binding properties.
• The human CDA monomer has a molecular weight of approximately 16 kDa (UniProt: 16185 Da).
• A three-dimensional structure is available, supporting structural and functional studies.

Experimental Guidance and Technical Tips

• The immunogen encompasses the full-length human CDA protein (amino acids 1-146), which may aid in the detection of both native and denatured forms.
• For IHC-P applications, consider using heat-induced antigen retrieval and validate in granulocyte-rich tissues (e.g., bone marrow or inflammatory lesions) using appropriate controls.
• For intracellular flow cytometry (FC), ensure thorough permeabilization; validate the staining in the target cell system and include isotype controls to confirm specificity.
• In ELISA, the antibody may be used as a capture or detection reagent; cross-reactivity with mouse CDA extends its utility to mouse model studies—validate in your specific assay format.
• Note the small molecular weight (~16 kDa) when optimizing transfer and detection conditions if adapting this antibody for western blotting.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to providing diagnostic manufacturers, laboratories, and research institutes with comprehensive access to high-quality IVD raw materials, technical services, and consulting. This Anti-CDA Rabbit Monoclonal Antibody supports research in pyrimidine metabolism and immune cell biology. As your one-stop sourcing partner, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets to accelerate your assay development from concept to clinic.