Anti-CDA Polyclonal Antibody for WB, IF/ICC, ELISA - P32320

Core Product Specifications and Parameters

Biological Background: Cytidine Deaminase (CDA) Function and Localization

• CDA (cytidine deaminase), also known as CDD or cytidine aminohydrolase, is an enzyme involved in pyrimidine salvage (Gene ID: 978).
• It catalyzes the hydrolytic deamination of cytidine and 2'-deoxycytidine to uridine and deoxyuridine, respectively, providing substrates for UMP synthesis.
• CDA functions as a zinc-dependent hydrolase, requiring the metal ion for catalytic activity.
• The enzyme is highly expressed in granulocytes, while expression is low or absent in fibroblasts, chondrocytes, monocytes, and T/B lymphocyte cell lines.
• The human CDA protein consists of 146 amino acids, with a predicted molecular weight of approximately 16 kDa.
• Structural studies have determined the three-dimensional conformation of CDA, revealing key active-site residues.
• Keywords associated with CDA include hydrolase, metal-binding, zinc, and reference proteome.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein covering the full-length human CDA sequence (aa 1-146). As a polyclonal antibody, it may detect multiple epitopes, offering robust signal in denaturing and native conditions.
• For Western blot, the predicted band size is ~16 kDa. Using reducing conditions and appropriate molecular weight markers is recommended. Consider validating the antibody in a relevant sample type, such as granulocyte lysates, which are known to highly express CDA.
• For immunofluorescence/ICC, optimize fixation and permeabilization steps, as CDA is a cytosolic protein. Include appropriate controls (e.g., knockdown or knockout cell lines) to confirm specificity.
• For ELISA, this antibody can be used as a capture or detection reagent. Perform checkerboard titrations to determine optimal working concentrations.
• Cross-reactivity with mouse and rat CDA has been reported, making this antibody suitable for studies across these species. However, it is advisable to confirm reactivity in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibodies, recombinant proteins, and ancillary reagents for diagnostic development. For researchers investigating nucleotide metabolism and pyrimidine salvage pathways, we provide reliable antibodies like this anti-CDA polyclonal antibody, along with technical support and bulk supply options to accelerate your assay development from concept to clinic.