Anti-CDA Monoclonal Antibody for WB, IHC-P, ELISA - P32320

Core Product Specifications and Parameters

Biological Background: CDA Function and Localization

• CDA (Cytidine deaminase), also known as CDD or cytidine aminohydrolase, is a zinc-dependent hydrolase that scavenges exogenous and endogenous cytidine and 2'-deoxycytidine, converting them to uridine and deoxyuridine for UMP synthesis.
• It plays a critical role in nucleotide metabolism and the pyrimidine salvage pathway.
• Highly expressed in granulocytes, with very low expression in fibroblasts, chondrocytes, monocytes, and T- and B-cell lines, suggesting a specialized function in myeloid cells.
• The enzyme requires zinc as a metal cofactor for its catalytic activity.
• Belongs to the cytidine and deoxycytidylate deaminase family; 3D structure has been determined.
• Predicted molecular weight of ~16 kDa.

Experimental Guidance and Technical Tips

• The immunogen used is full-length human CDA recombinant protein, so the antibody is expected to recognize the native protein across applications.
• For Western blotting, a band at ~16 kDa corresponding to CDA monomer may be observed; however, note that endogenous expression is tissue-specific, with high levels in granulocytes and low levels in other cell types.
• In IHC-P, consider using granulocyte-rich tissues (e.g., bone marrow, spleen) as positive controls, and validate staining with appropriate negative controls.
• The antibody cross-reacts with mouse CDA; for experiments using mouse samples, validate in the relevant mouse tissue/cell type, considering potential differences in expression patterns.
• ELISA applications may require optimization of coating antigen and antibody dilutions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. For studies in nucleotide metabolism and related pathways, this anti-CDA antibody is available alongside CamelBio's expertise in rare target sourcing and technical support, helping you move from concept to clinic efficiently.