Anti-CD99L2 Rabbit Polyclonal Antibody for WB - Q8TCZ2

Core Product Specifications and Parameters

Biological Background: CD99L2 Function and Localization

• CD99L2 (CD99 antigen-like protein 2), also known as MIC2-like protein 1 (MIC2L1) and CD99B, is a transmembrane glycoprotein belonging to the CD99 family.
• It plays a critical role in leukocyte extravasation by facilitating cell passage through the endothelial basement membrane, acting independently of PECAM1 (By similarity).
• CD99L2 exhibits homophilic adhesion properties, though these interactions may not be essential for cell aggregation (By similarity).
• The protein is localized to the cell membrane and cell junctions, and a secreted form has also been detected (UniProt subcellular location).
• CD99L2 is broadly expressed across many tissues, with lower expression in the thymus; it has been identified at the protein level in cerebrospinal fluid (PubMed:25326458). Related references: PMID:25326458
• Post-translational modifications include glycosylation and likely proteoglycan modifications, indicated by the presence of a signal peptide and transmembrane domain (UniProt keywords).
• Alternative splicing produces multiple isoforms, contributing to functional diversity (UniProt keyword).

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 26-185 of human CD99L2, which includes part of the extracellular domain; the polyclonal nature may recognize multiple epitopes, ensuring robust detection in WB and ELISA.
• For Western blot, consider using a positive control such as human, mouse, or rat tissue/cell lysates known to express CD99L2 (e.g., lung, heart, or brain lysates based on broad tissue expression).
• Predicted molecular weight is approximately 28 kDa, but glycosylation may cause a shift to higher apparent molecular weight; validate in your specific sample system.
• For ELISA, the antibody can be used as a capture or detection reagent; perform titration experiments to determine optimal coating concentration and detection sensitivity.
• Cross-reactivity with mouse and rat has been reported; ensure species-specific validation if working with these models, as homology may affect sensitivity.
• Since CD99L2 is also secreted, consider testing cell culture supernatants for ELISA applications if analyzing secreted forms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials, from validated antibody pairs to bulk ancillary reagents, supporting researchers and diagnostic developers in leukocyte adhesion and extravasation studies. Our one-stop sourcing platform streamlines procurement of high-quality antibodies like this anti-CD99L2 polyclonal antibody, and we can assist with rare target reagent sourcing for your custom assay development needs.