Anti-CD99 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P14209

Core Product Specifications and Parameters

Biological Background: CD99 Function and Localization

• CD99 (also known as MIC2, 12E7, E2 antigen) is a transmembrane glycoprotein encoded by the CD99 gene on the pseudoautosomal region of human sex chromosomes, with multiple alternatively spliced isoforms.
• Functions in T-cell adhesion processes and mediates spontaneous rosette formation with erythrocytes, contributing to immune cell interactions.
• Plays a critical role in leukocyte extravasation, facilitating leukocyte migration through the endothelial basement membrane independently of PECAM1.
• Subcellular localization is primarily at the plasma membrane, consistent with its role in cell adhesion and signaling.
• Post-translational modifications include glycosylation and phosphorylation, as indicated by UniProt keywords.
• The three-dimensional structure has been determined, providing insights into its molecular interactions.

Experimental Guidance and Technical Tips

• The immunogen (aa 23–185) corresponds to the extracellular domain; consider that the antibody may recognize surface CD99 in native conditions.
• For WB under reducing conditions, expect a band near 19 kDa; validate in the relevant sample system and use appropriate controls.
• For IHC-P and IF/ICC, optimize fixation/permeabilization as CD99 is a membrane protein; antigen retrieval may enhance detection.
• In ELISA applications, confirm suitability as capture or detection antibody through pilot experiments with known positive and negative samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting, supporting projects from concept to clinic. For CD99-related research in immunology and cell adhesion, we offer validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development and supply chain.