Anti-CD98/SLC3A2 Rabbit Monoclonal Antibody for Western Blot, Immunofluorescence, ELISA - P08195

Core Product Specifications and Parameters

Biological Background: CD98/SLC3A2 Function and Localization

• CD98/SLC3A2 (4F2 heavy chain) is a type II transmembrane glycoprotein that acts as a chaperone for SLC7 family light chains, forming functional heterodimeric amino acid transporters at the plasma membrane. Related references: PMID:10574970, PMID:10903140, PMID:11557028

• The SLC3A2/SLC7A5 (LAT1) and SLC3A2/SLC7A8 (LAT2) heterodimers mediate sodium-independent transport of large neutral amino acids, thyroid hormones, and L-DOPA, with SLC3A2 being essential for transporter stability and surface localization. Related references: PMID:10391915, PMID:10574970, PMID:11311135

• SLC3A2/SLC7A11 (xCT) functions as a cystine/glutamate antiporter, importing cystine for glutathione synthesis and protecting cells from oxidative stress. Related references: PMID:34880232

• CD98/SLC3A2 is a key modulator of integrin signaling, promoting cell adhesion, spreading, and migration, and contributes to tumor progression. Related references: PMID:11121428, PMID:15625115

• In lysosomes, SLC3A2 partners with LAPTM4B to deliver SLC7A5 to the lysosomal membrane, facilitating leucine uptake and mTORC1 activation. Related references: PMID:25998567

• The protein localizes to multiple membrane compartments: apical and basolateral cell membrane, cell junctions, lysosome membrane, and melanosome.

• Ubiquitously expressed in human tissues, with highest levels in kidney, placenta, and testis; also found in endothelial cells, lymphocytes, and various epithelial cell lines.

• Post-translational modifications include N-linked glycosylation, disulfide bond formation, acetylation, phosphorylation, and ubiquitination, as identified by proteomics.

• During hepatitis C virus infection, SLC3A2/SLC7A5 facilitates viral entry and enhances mTORC1 signaling via increased leucine uptake.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 461-630) of human CD98, which is predicted to be intracellular; this epitope may be less affected by glycosylation.
• For Western blotting, use reducing conditions; the core protein is ~58 kDa, but glycosylation can increase the apparent molecular weight. Validate using human tissue or cell line lysates.
• In immunofluorescence/immunocytochemistry, expect membrane and intracellular vesicular (lysosome) staining patterns. Permeabilization may be required for detection of intracellular epitopes.
• For ELISA, the antibody can be tested as a capture or detection reagent; pair with a suitable detection antibody for sandwich ELISA development.
• Cross-reactivity with human CD98 has been confirmed; test for cross-reactivity in other species if required for your assay.
• Always titrate the antibody for your specific application and validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories streamlined access to high-quality IVD raw materials. This anti-CD98/SLC3A2 rabbit monoclonal antibody is part of our portfolio supporting research on amino acid transport, cancer metabolism, and viral infection. Beyond this product, we can supply validated antibody pairs, bulk ancillary reagents, and assist with sourcing rare or custom raw materials—all backed by our integrated technical services to advance your assay development from concept to clinic.