Anti-CD98/SLC3A2 Monoclonal Antibody for WB, IF/ICC, ELISA - P08195 / P10852

Core Product Specifications and Parameters

Biological Background: CD98/SLC3A2 Function and Localization

• SLC3A2, also known as CD98 heavy chain or 4F2hc, is a type II transmembrane glycoprotein that serves as a chaperone for SLC7 family amino acid transporters, forming heterodimeric exchangers at the plasma membrane. Related references: PMID:10574970, PMID:10903140, PMID:11557028

• The substrate specificity of SLC3A2-SLC7 heterodimers depends on the SLC7 subunit; for example, SLC3A2/SLC7A5 transports large neutral amino acids, while SLC3A2/SLC7A11 exchanges cystine for glutamate, and SLC3A2/SLC7A6 or SLC7A7 mediate uptake of dibasic amino acids. Related references: PMID:10574970, PMID:10903140, PMID:11389679, PMID:11557028, PMID:9829974, PMID:34880232

• SLC3A2 is essential for the plasma membrane localization, stability, and transport activity of SLC7A5 and SLC7A8, and can also recruit SLC7A5 to lysosomes via LAPTM4B to promote leucine uptake and mTORC1 activation. Related references: PMID:10391915, PMID:10574970, PMID:11311135, PMID:25998567

• The protein localizes to the apical and basolateral cell membrane, cell junctions, lysosome membrane, and melanosomes, reflecting its broad functional distribution.

• CD98/SLC3A2 modulates integrin-related signaling and is essential for integrin-dependent cell spreading, migration, and tumor progression. Related references: PMID:11121428, PMID:15625115

• It is ubiquitously expressed, with highest levels in kidney, placenta, and testis, and is upregulated in various cancers and activated lymphocytes.

• Post-translational modifications include disulfide bond formation, N-glycosylation, phosphorylation, acetylation, and ubiquitination, which are critical for its function and trafficking.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 206–630 of the human/mouse SLC3A2 sequence, which encompasses a large intracellular region; thus, the antibody is likely to recognize both the reduced and non-reduced forms in WB. For optimal detection, run samples under reducing conditions and use a positive control such as HeLa or HEK293 cell lysates, where SLC3A2 is highly expressed.
• Given the reported cross-reactivity with rat, this antibody may be suitable for studies in rat tissues or cell lines, but independent validation is recommended to confirm specificity in the chosen experimental system.
• For immunofluorescence (IF/ICC), since SLC3A2 localizes to the plasma membrane and endolysosomal compartments, consider using permeabilization with 0.1% Triton X-100 and co-staining with organelle markers to assess subcellular localization.
• In ELISA applications, ensure that the recombinant protein or peptide used for coating exposes the epitope recognized by this antibody (aa 206–630). Optimize coating and blocking conditions to minimize background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories one-stop access to a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. To support your CD98/SLC3A2-related projects—whether for tumor biomarker research, amino acid transport studies, or infectious disease diagnostics—we can source rare specificities and deliver reliable, high-quality antibody raw materials tailored to your development pipeline. Partner with CamelBio to bridge the gap from target discovery to clinical application efficiently.