Anti-CD98 Monoclonal Antibody for FC - P10852

Core Product Specifications and Parameters

Biological Background: CD98 Function and Localization

• Protein identity and aliases: CD98 is the heavy chain of the amino acid transporter, also known as solute carrier family 3 member 2 (SLC3A2) or 4F2hc. It acts as a chaperone for SLC7 family light chains.
• Core amino acid transport function: CD98 forms heterodimers with various SLC7 transporters (e.g., SLC7A5, SLC7A6, SLC7A7, SLC7A8, SLC7A10, and SLC7A11) to mediate amino acid exchange across the plasma membrane. The substrate specificity depends on the SLC7A subunit; for example, SLC3A2/SLC7A11 exchanges extracellular cystine for intracellular glutamate, while SLC3A2/SLC7A5 transports large neutral amino acids, thyroid hormones, and L-DOPA. Related references: PMID:10574970, PMID:10734121, PMID:11011012
• Integrin signaling and cell migration: CD98 modulates integrin-dependent signaling and is essential for integrin-mediated cell spreading, migration, and tumor progression.
• Subcellular localization: CD98 localizes to the apical and basolateral cell membranes, cell junctions, lysosome membranes, and melanosomes, consistent with its role in amino acid transport and cellular adhesion.
• Tissue expression: CD98 is widely expressed in mouse tissues, with strongest expression in kidney, small intestine, spleen, thymus, and liver. It is also detected on embryonic epithelial cells and brain capillary endothelia. Related references: PMID:9915839
• Post-translational modifications: CD98 is a glycoprotein with disulfide bonds, phosphorylation sites, and isopeptide bond linkages. It undergoes ubiquitination and alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 105–526), making this antibody well-suited for detecting cell surface CD98 in flow cytometry applications.
• For flow cytometry, use viable cells or apply appropriate fixation/permeabilization protocols if intracellular staining is required, because CD98 is a transmembrane protein.
• Validate antibody performance in mouse cell lines or primary cells known to express CD98, such as activated lymphocytes, kidney epithelial cells, or intestinal epithelial cells.
• Titrate the antibody to determine the optimal staining concentration for your specific flow cytometry protocol and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-CD98 antibody supports research into amino acid transport and integrin-mediated signaling. Contact us to learn how we can streamline your diagnostic or research workflow from concept to clinic.