Anti-CD97 Rabbit Polyclonal Antibody for WB, ELISA - Q9Z0M6

Core Product Specifications and Parameters

Biological Background: CD97 Function and Localization

• CD97 (also known as Adhesion G protein-coupled receptor E5 or leukocyte antigen CD97) is encoded by the Adgre5 gene in mouse.
• It functions as a receptor involved in both adhesion and signaling processes, playing an essential role in leukocyte migration.
• CD97 belongs to the adhesion G protein-coupled receptor (GPCR) family, characterized by a large extracellular domain with EGF-like repeats that facilitate cell-cell interactions.
• At the protein level, CD97 is subject to alternative splicing, glycosylation, and phosphorylation, and it contains calcium-binding EGF-like domains and disulfide bonds critical for its structural integrity.
• Subcellularly, CD97 localizes to the cell membrane and can also be secreted into the extracellular space.
• Although predominantly expressed by immune cells, CD97 is ubiquitously found with particularly high expression in the lung and thymus gland; in spleen, it is detected on most myeloid cells and variable portions of T-cells, B-cells, and NK cells, while in bone marrow it is present in nearly all myeloid cells but largely absent from erythroid cells.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 24–265 of mouse CD97, covering part of the N-terminal extracellular region; this design may recognize both membrane-bound and soluble forms.
• For western blotting, an expected band around 90 kDa may be observed; due to glycosylation and potential disulfide-linked structures, consider running samples under both reducing and non-reducing conditions to resolve the target.
• For ELISA applications, the antibody can be used as a capture or detection reagent; optimize coating concentration and detect with appropriate secondary antibodies.
• Validation in relevant mouse tissue lysates (e.g., spleen, lung, or thymus) is recommended to confirm specificity and sensitivity in the experimental context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in connecting diagnostic developers and research laboratories with high-quality IVD raw materials, technical expertise, and supply-chain solutions from concept to clinic. This anti-CD97 polyclonal antibody is part of our extensive portfolio for cell adhesion, migration, and immunology research. Beyond this reagent, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets to streamline your assay development and manufacturing needs.