Anti-CD97 () Monoclonal Antibody for Flow Cytometry - P48960

Core Product Specifications and Parameters

Biological Background: CD97 Function and Localization

• CD97, also known as Adhesion G protein-coupled receptor E5 or Leukocyte antigen CD97, is encoded by the ADGRE5 gene.
• Functions as an adhesion G protein-coupled receptor involved in both adhesion and signaling processes early after leukocyte activation, playing an essential role in leukocyte migration.
• Contains EGF-like domains, a GPCR proteolytic site, and a transmembrane helix characteristic of the adhesion GPCR family.
• Localized predominantly at the cell membrane and is also found secreted in the extracellular space.
• Broadly expressed on most hematopoietic cells, including activated lymphocytes, monocytes, macrophages, dendritic cells, and granulocytes; also expressed by smooth muscle cells and various carcinomas.
• Expression is upregulated under inflammatory conditions, such as in the CNS of multiple sclerosis and synovial tissue of rheumatoid arthritis, with detectable soluble CD97 in synovial fluid.
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 21-398 of human CD97) encompasses the extracellular region, which is ideal for detecting cell surface expression in flow cytometry.
• Consider using appropriate isotype controls and Fc receptor blocking to minimize nonspecific binding when staining hematopoietic cells.
• Validate antibody performance in the relevant sample system (e.g., peripheral blood mononuclear cells, activated lymphocytes) to ensure specificity.
• For intracellular detection, permeabilization protocols may need optimization due to the transmembrane nature of CD97.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio facilitates one-stop sourcing of high-quality IVD raw materials, including validated antibodies for immune targets like CD97. With expertise in monoclonal antibody development and bulk reagent supply, CamelBio supports diagnostic manufacturers from concept to clinic, providing everything from optimized antibody pairs to ancillary reagents for rare-target applications.