Anti-CD97 (ADGRE5) Rabbit Polyclonal Antibody for WB and ELISA - P48960

Core Product Specifications and Parameters

Biological Background: CD97 Function and Localization

• CD97, also known as ADGRE5 or TM7LN1, is an adhesion G protein-coupled receptor (aGPCR) belonging to the EGF-TM7 subfamily. It mediates both cell adhesion and signaling upon leukocyte activation.
• The protein plays an essential role in leukocyte migration, facilitating immune cell trafficking to sites of inflammation.
• CD97 is expressed as a heterodimer consisting of a large extracellular α-subunit and a seven-span transmembrane β-subunit, which are non-covalently associated.
• Subcellularly, the receptor localizes to the cell membrane and can also be shed into the extracellular space, producing a soluble form.
• Tissue expression is broad: CD97 is found on most hematopoietic cells (including activated lymphocytes, monocytes, macrophages, dendritic cells, and granulocytes) and is abundantly expressed by smooth muscle cells.
• Expression is upregulated in various carcinomas (thyroid, colorectal, gastric, esophageal, pancreatic) and under inflammatory conditions, such as in the CNS of multiple sclerosis and synovial tissue of rheumatoid arthritis.
• Key structural and functional features include multiple EGF-like domains, N-glycosylation, disulfide bonds, and potential phosphorylation sites, consistent with its classification as a glycoprotein and phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 240-435 of human CD97, which covers the extracellular region containing EGF-like domains. This design is typical for generating antibodies that recognize native surface receptors and may be suitable for detecting both membrane-bound and soluble forms.
• Given the polyclonal nature of the antibody, it may recognize multiple epitopes, potentially enhancing sensitivity in Western blot (predicted MW ~92 kDa) and ELISA applications. However, lot-to-lot consistency should be verified for long-term studies.
• The antibody cross-reacts with mouse and rat CD97, enabling translational studies across species. Validate the reactivity and specificity in your target tissue or cell line, as expression levels can vary under different conditions.
• For ELISA, consider pairing with a detection antibody targeting a non-overlapping epitope if developing a sandwich assay. Soluble CD97 in biological fluids might be a biomarker for inflammatory diseases, so appropriate sample handling and assay validation are advisable.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibodies like this anti-CD97 polyclonal antibody. As a partner for leukocyte biology and inflammation-related target discovery, we streamline sourcing of high-quality antibody pairs, recombinant proteins, and ancillary reagents. Whether you need bulk quantities, customized conjugates, or rare target raw materials, CamelBio supports your project from concept to clinical application with reliable supply and technical consultation.