Anti-CD95/FAS Monoclonal Antibody for WB, IF/ICC, ELISA - P25445

Core Product Specifications and Parameters

Biological Background: CD95/FAS Function and Localization

• CD95/FAS is a member of the tumor necrosis factor receptor superfamily (TNFRSF6), also known as Apo-1 antigen or apoptosis-mediating surface antigen FAS.
• It functions as a receptor for TNFSF6/FASLG, and upon ligand binding, recruits FADD and caspase-8 to form the death-inducing signaling complex (DISC), initiating apoptosis.
• FAS-mediated apoptosis plays a role in peripheral tolerance induction and antigen-stimulated suicide of mature T-cells.
• The gene produces multiple isoforms via alternative splicing; secreted isoforms 2 to 6 can block apoptosis in vitro.
• Subcellular localization includes cell membrane, membrane rafts, and secreted forms; isoform-specific localization influences signaling.
• In resting peripheral blood mononuclear cells, isoform 1 and isoform 6 are equally expressed; upon activation, isoform 1 increases while isoform 6 decreases.
• Post-translational modifications include glycosylation, palmitoylation, phosphorylation, and disulfide bond formation, contributing to receptor function and stability.
• Mutations in FAS are associated with autoimmune lymphoproliferative syndrome (ALPS), highlighting its essential role in immune homeostasis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 26-173), so the antibody is expected to recognize the membrane-bound and possibly secreted isoforms of CD95/FAS.
• For Western blot, anticipate multiple bands between 9-38 kDa due to alternative splicing, glycosylation, and cleavage; consider using reducing conditions and appropriate molecular weight markers.
• In IF/ICC, CD95/FAS localizes primarily to the plasma membrane and membrane rafts; ensure permeabilization optimization if detecting intracellular pools.
• Validate the antibody in your specific sample system, as expression levels may vary with cell type and activation state.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research institutes with essential IVD raw materials, offering a streamlined sourcing pathway from concept to clinic. This CD95/FAS monoclonal antibody supports apoptosis and immune function studies, and CamelBio’s comprehensive portfolio includes validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and rare-target raw-material procurement to accelerate your assay development.