Anti-CD95/FAS Monoclonal Antibody () for Flow Cytometry - P25446

Core Product Specifications and Parameters

Biological Background: CD95/FAS Function and Localization

• CD95 (FAS) is a transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily, encoded by the Fas gene (also known as Apt1 or Tnfrsf6) in mouse.
• It functions as the receptor for the FAS ligand (TNFSF6/FASLG) and plays a central role in the extrinsic apoptotic pathway through formation of the death-inducing signaling complex (DISC).
• Upon ligand binding, the adapter molecule FADD recruits caspase-8, leading to its proteolytic activation and subsequent caspase cascade, ultimately triggering apoptosis.
• FAS-mediated apoptosis is implicated in the induction of peripheral tolerance and in the antigen-stimulated suicide of mature T-cells.
• The protein is localized to the cell membrane and enriched in membrane rafts, with tissue expression detected in thymus, liver, lung, heart, and adult ovary.
• Post-translational modifications include N-linked glycosylation, palmitoylation, and phosphorylation; it also contains calmodulin-binding motifs and disulfide bonds critical for structural integrity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 22–169 of mouse CD95/FAS, covering the extracellular domain; the resulting antibody may recognize epitopes within this region.
• As a directly conjugated antibody (), it is ready for use in flow cytometry without additional secondary staining steps.
• When establishing the assay, consider titration of the antibody to determine optimal staining concentration for your specific cell type.
• For best results, validate the antibody in mouse tissue samples known to express CD95 (e.g., thymus, liver, lung) using appropriate positive and negative controls.
• This antibody has been reported for use in flow cytometry; applicability to other applications such as IHC or WB should be tested experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. For apoptosis and immunology research, CamelBio offers expert sourcing of rare target raw materials, ensuring you can obtain the critical reagents needed without supply chain delays. Partner with us to accelerate your CD95/FAS assay development and scale your projects from concept to clinic.