Anti-CD95 Rabbit Monoclonal Antibody for ELISA, FC - P25446

Core Product Specifications and Parameters

Biological Background: CD95 Function and Localization

• CD95 (FAS, Apo-1) is a member of the tumor necrosis factor receptor superfamily, a transmembrane protein that mediates apoptosis upon binding to its ligand FASLG.
• The receptor's intracellular death domain recruits FADD and caspase-8, forming the death-inducing signaling complex (DISC) that triggers apoptotic cascades.
• FAS-mediated apoptosis is critical for peripheral tolerance and the antigen-stimulated suicide of mature T-cells.
• Localized to the cell membrane and membrane rafts, CD95 is expressed in various mouse tissues including thymus, liver, lung, heart, and adult ovary.
• Post-translational modifications include glycosylation, lipidation (palmitate), and phosphorylation; the protein contains disulfide bonds and a signal peptide.
• The mature protein has a predicted molecular weight of approximately 37 kDa, which may appear higher under reducing conditions due to glycosylation.

Experimental Guidance and Technical Tips

• The immunogen (aa 22-169) spans the extracellular domain, making this antibody suitable for detecting surface CD95 in native and denaturing conditions.
• For ELISA, consider coating with recombinant CD95-Fc fusion or using cell lysates; titrate the antibody from 0.1-1 µg/mL as a starting point.
• In flow cytometry, use 0.5-2 µg per 10^6 cells; include appropriate isotype controls and blocking steps to minimize nonspecific binding.
• Validate antibody specificity using CD95 knockout mouse models or cell lines to confirm target recognition.
• Note that endogenous CD95 expression levels vary between tissues and activation states; pilot experiments are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials, including validated monoclonal antibodies like this anti-CD95 clone, to support apoptosis research and diagnostic development. We offer bulk quantities, custom formulations, and technical consultation to streamline your assay optimization. Whether you need matched antibody pairs, rare target reagents, or ancillary proteins, CamelBio bridges your sourcing needs from concept to clinic.