Anti-CD94 Monoclonal Antibody for FC - Q13241

Core Product Specifications and Parameters

Biological Background: CD94 Function and Localization

• CD94, encoded by KLRD1, is a transmembrane glycoprotein and C-type lectin receptor serving as a ligand-binding subunit in heterodimeric complexes with KLRC1 (NKG2A) or KLRC2 (NKG2C).
• In the KLRD1-KLRC1 complex, it functions as a key inhibitory receptor on NK cells and a subset of CD8+ T cells, recognizing HLA-E loaded with self-peptides to monitor MHC class I expression and maintain self-tolerance.
  Related references: PMID:30860984, PMID:9430220, PMID:9485206, PMID:12387742, PMID:18064301, PMID:30503213, PMID:12165520
• In the KLRD1-KLRC2 complex, it acts as an activating receptor, recognizing HLA-E bound to peptides from HLA-G, contributing to adaptive NK cell responses and maternal-fetal tolerance.
  Related references: PMID:15940674, PMID:9655483, PMID:30134159, PMID:9754572, PMID:20952657, PMID:21825173
• CD94 is localized to the cell membrane and is predominantly expressed in NK cell subsets and terminally differentiated cytotoxic T cells, with differential expression of the inhibitory and activating complexes.
  Related references: PMID:21825173, PMID:9430220, PMID:9485206, PMID:12387742, PMID:20952657
• Post-translational modifications include N-linked glycosylation and disulfide bond formation; alternative splicing generates multiple isoforms.
• During viral infections (e.g., CMV, HIV, SARS-CoV-2), CD94 recognizes HLA-E presenting viral peptides, contributing to immune evasion and NK cell exhaustion.
  Related references: PMID:32859121

Experimental Guidance and Technical Tips

• The recombinant full-length human CD94 immunogen used for antibody generation suggests recognition of native conformational epitopes; confirm performance in relevant sample systems by pilot testing.
• For flow cytometry, employ standard staining protocols with appropriate isotype control and Fc receptor blocking to minimize nonspecific binding. Titrate the antibody to determine the optimal concentration for your cells.
• As CD94 is expressed on NK and T cell subsets, include lineage markers (e.g., CD3, CD56) for comprehensive immune phenotyping.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, offering validated monoclonal and polyclonal antibodies, antibody pairs, and bulk ancillary reagents to support diagnostic development. For CD94-targeted research, we provide ready-to-use conjugated antibodies like this format and can assist with custom sourcing of rare specificities and large-scale production needs.