Anti-CD93 Rabbit Monoclonal Antibody for IF/ICC, FC, ELISA - O89103

Core Product Specifications and Parameters

Biological Background: CD93 Function and Localization

• CD93 is a type I transmembrane glycoprotein also known as complement component C1q receptor, lymphocyte antigen 68 (Ly-68), AA4, C1qRp, and C1qr1. It belongs to the group of defense collagen receptors and contains EGF-like domains, glycosylation sites, and disulfide bonds.
• Acts as a cell surface receptor involved in various physiological processes, including inflammation, phagocytosis, and cell adhesion, and plays a key role in the immune response. Related references: PMID:15004139 PMID:20439917 PMID:38441970
• Functions as a phagocytic receptor by binding defense collagens such as C1q, mannose-binding lectin (MBL2), and surfactant protein A (SFTPA1), thereby enhancing the clearance of apoptotic cells and immune complexes. Related references: PMID:15004139
• Plays a critical role in angiogenesis and endothelial cell function by associating with beta-dystroglycan, leading to SRC-dependent phosphorylation and recruitment of CBL, which then provides a docking site for downstream signaling components like CRKL to promote cell migration. It also serves as a receptor for extracellular matrix ligands multimerin-2 (MMRN2) and IGFBP7.
• Regulates endothelial barrier integrity by modulating VEGFR2 signaling, thereby controlling vascular permeability. Related references: PMID:38441970
• Predominantly localized to the cell membrane; expressed in lung, heart, bone marrow, and macrophages, with lower levels in ovary and embryo, but absent in brain, adult liver, thymus, and epithelial cells.
• The predicted molecular weight is approximately 69 kDa, consistent with the observed 69 kDa band in SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 23–181 of mouse CD93, which corresponds to the N-terminal extracellular domain containing the C1q binding region and EGF-like repeats; this region is expected to be exposed on the cell surface and may also be present in soluble forms.
• For flow cytometry (FC), consider using this antibody to detect cell surface CD93, as the target is a transmembrane receptor. Validation in relevant cell lines or primary macrophages is recommended.
• In immunofluorescence/immunocytochemistry (IF/ICC), membrane staining should be observed; ensure appropriate fixation and permeabilization conditions to preserve epitope integrity.
• For ELISA applications, the recombinant immunogen may serve as a capture standard, and the antibody can be used for sandwich assays if paired with a suitable detection antibody. Sensitivity to the native protein should be confirmed.
• Cross-reactivity with human CD93 indicates potential utility in human cell systems; however, affinity and specificity should be verified in each experimental context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive partner for IVD raw-material sourcing, connecting you with high-quality reagents from research to clinical application. With this CD93 antibody, supporting inflammation, phagocytosis, and angiogenesis studies, CamelBio provides access to validated monoclonal and polyclonal antibodies, optimized antibody pairs, and bulk ancillary reagents for your diagnostic development. Whether you need rare target raw materials or routine consumables, CamelBio streamlines your procurement with reliable supply and expert consultation.