Anti-CD90/Thy1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P04216

Core Product Specifications and Parameters

Biological Background: CD90/Thy1 Function and Localization

• Encodes Thy-1 membrane glycoprotein, also known as CDw90, a member of the immunoglobulin superfamily.
• Functions in cell-cell and cell-ligand interactions, particularly during synaptogenesis and brain development.
• Localizes to the outer leaflet of the cell membrane via a GPI (glycosylphosphatidylinositol) anchor.
• Contains a single immunoglobulin-like domain, stabilized by a conserved disulfide bond.
• Post-translationally modified: N-glycosylated, phosphorylated, and contains a pyrrolidone carboxylic acid modification at the N-terminus.
• The protein has a predicted molecular weight of approximately 18 kDa, consistent with its mature form after signal peptide cleavage.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 20–130 of human CD90, covering the extracellular immunoglobulin domain; the antibody may recognize the native membrane-bound form.
• For Western blotting, expect a band around 18–25 kDa due to glycosylation; validate with positive controls such as brain or immune cell lysates.
• In immunofluorescence/immunocytochemistry, surface staining can be performed without permeabilization, but permeabilization may also allow intracellular detection if applicable.
• ELISA applications may require optimization of coating antigen and antibody concentration.
• Cross-reactivity with mouse and rat implies conservation of the epitope; suitability should be experimentally confirmed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines access to high-quality IVD raw materials, including validated polyclonal antibodies like anti-CD90/Thy1. For projects targeting this GPI-anchored membrane protein involved in cell adhesion and synaptogenesis, we offer sourcing services for bulk ancillary reagents and rare-target raw materials, supporting diagnostic developers from concept to clinic.