Anti-CD90/Thy1 Monoclonal Antibody (KD Validated) for WB, FC, ELISA - P04216

Core Product Specifications and Parameters

Biological Background: CD90/Thy1 Function and Localization

• CD90/Thy1, also known as CDw90 or Thy-1 antigen, is a GPI-anchored cell surface glycoprotein encoded by the THY1 gene (GeneID: 7070).
• It belongs to the immunoglobulin superfamily and contains a single Ig-like domain in its extracellular region.
• The protein localizes to the cell membrane and is attached via a glycosylphosphatidylinositol (GPI) anchor, lacking a transmembrane domain.
• Post-translational modifications include N-linked glycosylation, phosphorylation, a disulfide bond, and an N-terminal pyroglutamic acid residue.
• Its molecular weight is approximately 18 kDa, consistent with the predicted mass of 17935 Da for the mature peptide.
• Functionally, CD90/Thy1 is implicated in cell-cell and cell-ligand interactions, particularly during synaptogenesis and other events in the brain.
• It is expressed in various tissues, with well-documented roles in the nervous system and immune cell adhesion.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 20-130), making this antibody suitable for detecting CD90 on live cells in flow cytometry.
• For western blot, expect a band at ~18 kDa under reducing conditions; consider testing non-reducing conditions to assess the impact of the native disulfide bond.
• This clone has been validated by knockout/knockdown (KD) experiments, enhancing confidence in target specificity. However, it is advisable to validate the antibody in your experimental system with appropriate positive and negative controls.
• Optimize antibody dilutions for each application using a known positive sample, such as human brain lysate or CD90-expressing cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials and technical services. In the area of neuroscience and cell surface markers, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Whether you require standard catalog products or assistance with rare target raw-material sourcing, CamelBio is your one-stop partner from concept to clinic.