Anti-CD90/Thy1 Monoclonal Antibody for WB, IHC-P, ELISA - P04216

Core Product Specifications and Parameters

Biological Background: CD90/Thy1 Function and Localization

• CD90 (Thy-1) is a cell surface glycoprotein also designated CDw90, encoded by the THY1 gene in humans (UniProt P04216).
• It belongs to the immunoglobulin superfamily, characterized by an immunoglobulin-like domain, and is anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor.
• The protein undergoes several post-translational modifications, including N-linked glycosylation, disulfide bond formation, phosphorylation, and the addition of a GPI anchor; the signal peptide is cleaved and the N-terminal glutamine cyclized to pyroglutamic acid.
• Subcellularly, CD90 is localized to the cell membrane where it may participate in cell-cell and cell-ligand interactions.
• Functional studies suggest a role in synaptogenesis and other events in the brain, potentially mediating adhesion and signaling between neurons and glial cells.
• The mature protein has a predicted molecular weight of approximately 18 kDa.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide corresponding to the N-terminal region (aa 1-100) of human CD90. When planning experiments, consider that the antibody may recognize epitopes within this region.
• For western blot (WB), the reported molecular weight is ~18 kDa. Use appropriate gel percentages and transfer conditions for small proteins. Include positive controls such as human or rat brain lysates.
• In immunohistochemistry (IHC-P), antigen retrieval conditions may require optimization; test both heat-induced and enzymatic methods on formalin-fixed paraffin-embedded sections.
• For ELISA, the antibody can be used as a capture or detection reagent; validate the assay with recombinant CD90 protein or cell lysates.
• This antibody cross-reacts with rat CD90, enabling comparative studies in rodent models. Validate specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your diagnostic development by providing a comprehensive portfolio of high-quality IVD raw materials, including validated antibodies like this anti-CD90 clone. We support neuroscience and immunology research with ready-to-use monoclonal antibodies, recombinant proteins, and ancillary reagents. From concept to clinic, trust CamelBio for reliable, batch-to-batch consistent raw materials and expert technical consultation.