Anti-CD90.1 Rabbit Polyclonal Antibody for WB, ELISA - P01831

Core Product Specifications and Parameters

Biological Background: Thy-1 membrane glycoprotein Function and Localization

• Thy-1 membrane glycoprotein, also known as CD90, is a GPI-anchored cell surface protein belonging to the immunoglobulin superfamily.
• In mice, Thy-1 exists as two major allelic variants, Thy1.1 (CD90.1) and Thy1.2 (CD90.2); this antibody targets the CD90.1 allele as indicated by its immunogen design.
• Thy-1 may mediate cell-cell or cell-ligand interactions, particularly during synaptogenesis and other processes in the brain (based on UniProt functional annotation).
• The protein is localized to the cell membrane and is attached via a glycosylphosphatidylinositol (GPI) anchor, which targets it to lipid rafts.
• It contains a single immunoglobulin V-type domain, features a disulfide bond, and undergoes post-translational modifications including glycosylation and N-terminal pyrrolidone carboxylic acid formation.
• The mature protein has a molecular weight of approximately 18 kDa (based on UniProt entry), consistent with the observed ~18 kDa band in SDS-PAGE under reducing conditions.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was raised against a recombinant fusion protein covering amino acids 20-120 of mouse CD90.1 (NP_033408.1), which encompasses the extracellular immunoglobulin-like domain.
• For Western blotting (WB), a band of ~18 kDa is expected in mouse tissues or cell lines expressing CD90.1; inclusion of appropriate positive and negative controls (e.g., tissues from Thy1.1 vs. Thy1.2 mouse strains) is recommended.
• For ELISA, the antibody can be used as a capture or detection antibody; optimal coating concentrations and titers should be determined empirically.
• Polyclonal antibodies may exhibit lot-to-lot variability; we recommend validating each new lot in the intended assay system.
• Cell surface localization of Thy-1 via GPI anchor may require gentle lysis conditions to preserve solubility; consider using non-denaturing detergents if membrane fractionation is performed.
• Antibody specificity can be confirmed by immunoprecipitation coupled with mass spectrometry or by testing on lysates from CD90.1-knockout cells/tissues if available.
• Store as recommended (ice bag shipping; long-term storage at -20°C or -80°C) and avoid repeated freeze-thaw cycles to maintain activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a comprehensive portfolio of IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. This anti-CD90.1 rabbit polyclonal antibody, suited for WB and ELISA, supports neuroscience and immunology research involving mouse Thy1.1 allele expression. Whether you need optimized monoclonal or polyclonal antibodies, bulk reagent supply, or assistance with rare-target raw material sourcing, CamelBio serves as your reliable partner from concept to clinic.