Anti-CD9 Rabbit Monoclonal Antibody for WB, IF, IHC-P, FC, ELISA - P21926

Core Product Specifications and Parameters

Biological Background: CD9 Function and Localization

• CD9 (also known as MIC3, MRP-1, TSPAN29) is a member of the tetraspanin superfamily, a group of four-transmembrane domain proteins that organize membrane microdomains and modulate cell adhesion, migration, and signaling.
• CD9 is an integral membrane protein that associates with integrins and other tetraspanins to regulate diverse processes, including sperm-egg fusion, platelet activation and aggregation, and cell adhesion (PubMed:14575715, PubMed:18541721, PubMed:8478605). Related references: PMID:14575715, PMID:18541721, PMID:8478605
• In macrophages, CD9 associates with CD81 and beta-1/beta-2 integrins to prevent macrophage fusion into multinucleated giant cells specialized for ingesting complement-opsonized large particles (PubMed:12796480). Related references: PMID:12796480
• CD9 is involved in inhibiting myotube fusion during muscle regeneration (by similarity) and prevents the fusion of mononuclear cell progenitors into osteoclasts responsible for bone resorption (by similarity).
• CD9 acts as a receptor for PSG17 (by similarity) and regulates paranodal junction formation (by similarity).
• The CD9 protein contains disulfide bonds, is palmitoylated on juxtamembrane cysteine residues, and is annotated as a glycoprotein—features that contribute to its membrane organization and intermolecular interactions.
• Subcellularly, CD9 localizes to the cell membrane, is secreted in extracellular exosomes, and is detected in platelets as well as various hematopoietic and epithelial cells (PubMed:19640571). Related references: PMID:19640571
• CD9 plays a role in tumor metastasis and cell motility, with implications in cancer biology (PubMed:7511626, PubMed:8478605). Related references: PMID:7511626, PMID:8478605

Experimental Guidance and Technical Tips

• The immunogen spans the full-length human CD9 protein (amino acids 1–228), which may allow the antibody to recognize both extracellular and intracellular epitopes; perform epitope mapping if domain-specific binding is required.
• For Western blot, a band near 25 kDa is expected, though post‑translational modifications such as glycosylation and palmitoylation may cause a molecular weight shift; validate with appropriate positive and negative controls.
• In immunofluorescence and immunohistochemistry, CD9 is expressed on the plasma membrane and in intracellular vesicles; include permeabilization steps for intracellular staining, and consider co‑staining with organelle markers to confirm localization.
• For flow cytometry, use human cell lines or primary cells known to express CD9 (e.g., platelets, hematopoietic cells) as positive controls; Fc receptor blocking can reduce non‑specific binding.
• This antibody is suitable for ELISA; for sandwich ELISA, consider pairing it with a detection antibody that targets a non‑overlapping epitope.

CamelBio: Your One-Stop Sourcing Bridge

As your one-stop IVD raw‑material sourcing partner, CamelBio supplies high‑quality monoclonal antibodies such as this anti‑CD9 rabbit mAb to support research in cell adhesion, immunology, and cancer biology. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw‑material sourcing, helping diagnostic manufacturers accelerate development from concept to clinic.