Anti-CD8β Rabbit Monoclonal Antibody for WB, FC, ELISA - P10966

Core Product Specifications and Parameters

Biological Background: CD8β Function and Localization

• CD8β (T-cell surface glycoprotein CD8 beta chain, CD8B, LYT3) is a ~24 kDa single-pass type I membrane glycoprotein essential for adaptive immunity.
• Acts as a coreceptor with the T-cell receptor (TCR) for MHC class I:peptide complexes, recruiting the Src kinase LCK to initiate TCR signaling, leading to T-cell activation, lymphokine production, adhesion, and cytotoxic activity.
• CD8β forms heterodimers with CD8α; palmitoylation at its cytoplasmic tail targets CD8 to plasma membrane lipid rafts where signaling proteins are enriched.
• Subcellular localization includes cell membrane and secreted forms arising from alternative splicing.
• Multiple isoforms (1, 3, 5, 6, 7, 8) are expressed in thymus and peripheral CD8+ T-cells; isoform 1 is enriched in thymus, while isoform 6 predominates in peripheral T-cells.
• Post-translational modifications include disulfide bond formation, N-linked glycosylation, phosphorylation, and palmitoylation.
• Keywords: Adaptive immunity, Alternative splicing, Cell membrane, Disulfide bond, Glycoprotein, Immunity, Immunoglobulin domain, Lipoprotein, Membrane, Palmitate, Phosphoprotein, Secreted, Signal, Transmembrane, Transmembrane helix.

Experimental Guidance and Technical Tips

• For Western blotting, consider using 5% BSA blocking and detecting a ~24 kDa band in human peripheral blood lymphocyte lysates. Optimize primary antibody dilution empirically.
• In flow cytometry, use a fluorophore-conjugated anti-rabbit secondary antibody; titrate to determine optimal staining concentration for cell surface CD8β on human T-cells.
• For ELISA, this antibody can be used as a detection or capture reagent; validate pairing with a compatible partner antibody when developing a sandwich assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, technical services, and consulting. For CD8β-targeted immunoassay development and T-cell immunology research, we provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your diagnostic or research programs from concept to clinic.