Anti-CD8B Polyclonal Antibody for WB, ELISA - P10966

Core Product Specifications and Parameters

Biological Background: CD8B Function and Localization

• CD8B (CD8beta) is the beta chain of the CD8 coreceptor, an immunoglobulin domain-containing transmembrane glycoprotein essential for adaptive immunity.
• It functions as a coreceptor for MHC class I molecules, interacting simultaneously with the T-cell receptor (TCR) and the peptide-MHC complex on antigen-presenting cells.
• Upon engagement, the cytoplasmic tail recruits the Src kinase LCK to the TCR-CD3 complex, initiating downstream signaling cascades that promote cytokine production, adhesion, and cytotoxic T-lymphocyte activation.
• Palmitoylation of the cytoplasmic tail targets CD8B to lipid rafts, enriching signaling proteins and enhancing activation efficiency.
• CD8B is critically required for thymic selection and maturation of CD8+ T-cells.
• Multiple isoforms (1, 3, 5, 6, 7, 8) are generated by alternative splicing and expressed in both thymic and peripheral CD8+ T-cells, with isoform 1 predominant in thymus and isoform 6 in periphery.
• The protein localizes to the cell membrane and can also be secreted.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 22-170), making this antibody suitable for detecting both native and denatured CD8B.
• For Western blot, use whole cell lysates from T-cell lines (e.g., Jurkat) as positive control; a band of approximately 24 kDa is expected. Optimize antibody concentration by testing a dilution series.
• For ELISA, the recombinant immunogen protein can serve as a positive control to validate binding specificity and sensitivity.
• Cross-reactivity with mouse and rat CD8B is reported; however, endogenous expression levels may vary across tissues, so validation in the relevant sample system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio accelerates diagnostic development by providing a single entry point for IVD raw materials, technical support, and consulting—from target discovery to clinical application. This anti-CD8B polyclonal antibody supports T-cell biology and immunooncology research; we also offer matched antibody pairs, optimized monoclonal alternatives, and bulk ancillary reagents for immunoassay platforms. For rare or custom targets, our extensive sourcing network and production capabilities can be tailored to your project requirements.