Anti-CD8A Rabbit PolymAb® for WB, IHC-P, mIHC - P01732 / P01731

Core Product Specifications and Parameters

Biological Background: CD8A Function and Localization

• CD8A encodes the alpha chain of the T-cell surface glycoprotein CD8, which belongs to the immunoglobulin superfamily and is alternatively spliced.
• It functions as a coreceptor for MHC class I molecules on antigen-presenting cells, interacting simultaneously with the TCR and MHC class I to facilitate T-cell activation.
• Upon antigen recognition, CD8A recruits the Src kinase LCK to the TCR-CD3 complex, initiating downstream signaling pathways that lead to lymphokine production, motility, adhesion, and cytotoxic T-lymphocyte (CTL) activation.
• CD8A can form heterodimers with CD8B on thymus-derived T-cells or homodimers on subsets of natural killer cells, memory T-cells, intraepithelial lymphocytes, monocytes, and dendritic cells.
• In NK cells, CD8A homodimers at the cell surface promote survival and enable conjugation and lysis of multiple target cells.
• CD8A is localized primarily on the cell membrane but can also be secreted.
• Expressed in plasmacytoid dendritic cells upon herpes simplex virus-1 stimulation, enhancing immune surveillance.
• The protein is modified by disulfide bonds, glycosylation, and palmitoylation, reflecting its integral membrane nature.

Experimental Guidance and Technical Tips

• The immunogen comprising the extracellular domain (aa 22-182) suggests the antibody likely recognizes the native conformation of CD8A, making it suitable for applications that require detecting cell surface proteins, such as IF-P and IHC-P.
• For Western blotting, consider the heterodimeric nature of CD8, as multiple bands around 21-30 kDa may be observed due to glycosylation or dimerization; validate in relevant cell lysates.
• When used in multiplex immunohistochemistry (mIHC), consider pairing with other immune cell markers to profile T-cell subsets in tissue sections.
• Due to cross-reactivity among human, mouse, rat, and cynomolgus monkey, this antibody can be utilized for translational studies; however, always verify species-specific reactivity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-CD8A Rabbit PolymAb® is a high-quality rabbit monoclonal antibody targeting the CD8A coreceptor, essential for T-cell biology and immuno-oncology research. CamelBio supports your workflow with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your IVD development.