Anti-CD8A Rabbit Polyclonal Antibody for WB and ELISA - P01731

Core Product Specifications and Parameters

Biological Background: CD8A Function and Localization

• CD8A is the alpha chain of the CD8 coreceptor, also known as Lyt-2 or Ly-2, and belongs to the immunoglobulin superfamily.
• It functions as a coreceptor for MHC class I molecules by binding to the α3 domain of MHC I while simultaneously interacting with the T-cell receptor (TCR) on cytotoxic T lymphocytes.
• In T cells, CD8A recruits the Src kinase LCK to the TCR-CD3 complex, initiating phosphorylation cascades that lead to lymphokine production, adhesion, motility, and activation of cytotoxic T-lymphocytes (CTLs).
• In NK cells, CD8A homodimers provide a survival mechanism that enables conjugation and lysis of multiple target cells, and promote differentiation of activated lymphocytes into memory CD8 T cells.
• CD8A is located on the cell membrane and contains a transmembrane helix, an immunoglobulin domain, and is subject to palmitoylation and glycosylation.
• Alternative splicing of Cd8a generates multiple isoforms, contributing to the diversity of immune responses.
• UniProt keywords include adaptive immunity, disulfide bond, glycoprotein, lipoprotein, and signal peptide, highlighting its multifaceted role in immune function.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant mouse CD8A protein. For Western blot, the predicted molecular weight is 27 kDa; however, glycosylation may cause a slight shift. Validate in mouse immune tissues such as spleen or thymus.
• For ELISA, the antibody can serve as a capture or detection reagent; pairing with a suitable partner should be optimized experimentally.
• Cross-reactivity has only been confirmed for mouse; if using on samples from other species, verify specificity independently.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive supplier for IVD raw materials, offering validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents. This CD8A antibody supports immune-oncology and infectious disease research, and we facilitate sourcing of rare target reagents along with technical consultation—covering your needs from concept to clinic.