Anti-CD8A Polyclonal Antibody for WB, IHC-P, ELISA - P01732

Core Product Specifications and Parameters

Biological Background: CD8A Function and Localization

• CD8A encodes the alpha chain of the T-cell surface glycoprotein CD8, also known as T8/Leu-2, which is a coreceptor for MHC class I molecules. The CD8 protein exists as either a disulfide-linked heterodimer of alpha/CD8A and beta/CD8B chains or less commonly as a CD8A homodimer.
• CD8A is essential for the immune response. In T-cells, it interacts with the T-cell receptor (TCR) and MHC class I:peptide complexes on antigen-presenting cells, recruiting the Src kinase LCK to initiate signaling pathways that lead to T-cell activation, proliferation, and differentiation into cytotoxic T-lymphocytes (CTLs).
• CD8A enables CTLs to recognize and eliminate infected and tumor cells. The intracellular signaling cascade triggered by CD8A-LCK phosphorylation promotes lymphokine production, motility, adhesion, and target cell lysis.
• On natural killer (NK) cells, CD8A homodimers provide a survival mechanism that facilitates conjugation with and lysis of multiple target cells. CD8A homodimers also support the survival and differentiation of activated lymphocytes into memory CD8+ T-cells.
• CD8A is expressed on thymus-derived T-cells, a subset of NK cells, memory T-cells, intraepithelial lymphocytes, monocytes, and dendritic cells. Its expression can be induced on plasmacytoid dendritic cells upon herpes simplex virus-1 stimulation.
• The protein is an integral membrane glycoprotein with a single-pass transmembrane domain and a secreted form produced by alternative splicing. It contains an immunoglobulin-like domain and is modified by disulfide bonds, palmitoylation, and N-linked glycosylation.
• Keywords associated with CD8A include adaptive immunity, alternative splicing, cell membrane, disease variant (implicating it in immunodeficiency), disulfide bond, glycoprotein, immunoglobulin domain, lipoprotein, palmitate, secreted, and transmembrane helix.
• The molecular weight of the mature protein is approximately 25.7 kDa, consistent with the observed band size of 26 kDa under reducing conditions in Western blot analysis.

Experimental Guidance and Technical Tips

• The immunogen used to generate this polyclonal antibody corresponds to amino acids 22-182 of human CD8A, which spans the extracellular immunoglobulin-like domain. This region is highly likely to be accessible on the cell surface, making the antibody suitable for applications recognizing native protein.
• For Western blot (WB), a band around 26 kDa is expected. The antibody may detect both glycosylated and unglycosylated forms. Reducing conditions are recommended to resolve the monomer.
• For immunohistochemistry on paraffin-embedded sections (IHC-P), optimal antigen retrieval conditions (e.g., citrate buffer, pH 6.0) should be determined empirically. The polyclonal nature may provide strong signal amplification but could also increase background; blocking steps should be optimized.
• As a polyclonal antibody, it recognizes multiple epitopes, which can be advantageous for ELISA assays, potentially serving as both a capture and detection reagent. Cross-adsorption against mouse proteins ensures reactivity with mouse CD8A, expanding its utility in mouse model studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing platform for IVD raw materials, bridging diagnostic manufacturers and laboratories with high-quality reagents like this anti-CD8A antibody. Whether you need validated antibody pairs, bulk monoclonals or polyclonals, or ancillary reagents for T-cell immunology research, CamelBio streamlines procurement and offers technical consultation. Our portfolio covers everything from concept to clinic, including rare target antibody sourcing, to accelerate your assay development.