Anti-CD8a Monoclonal Antibody for Flow Cytometry - P01732

Core Product Specifications and Parameters

Biological Background: CD8a Function and Localization

• CD8a is a T-cell surface glycoprotein that serves as a coreceptor for MHC class I molecules, enabling cytotoxic T-lymphocytes to recognize and eliminate infected or tumor cells.
• It exists as either a heterodimer with CD8B or a homodimer, and is expressed on thymocytes, cytotoxic T-cells, NK cells, memory T-cells, and intraepithelial lymphocytes.
• CD8a simultaneously engages the T-cell receptor (TCR) and MHC class I proteins, recruiting the LCK kinase to phosphorylate signaling substrates for T-cell activation, cytokine production, and adhesion.
• In NK-cells, CD8A homodimers support survival and serial killing of target cells, while in activated lymphocytes they promote differentiation into memory CD8+ T-cells.
• The protein is a single-pass type I membrane protein, but a secreted isoform exists; alternative splicing generates multiple transcripts.
• Post-translational modifications include disulfide bond formation, N-glycosylation, and palmitoylation, which anchor it to lipid rafts.
• Tissue specificity: expressed on a subset of immune cells, and upregulated on plasmacytoid dendritic cells upon herpes simplex virus-1 stimulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 22–182) of human CD8A, making this antibody suitable for detecting surface CD8a by flow cytometry.
• For flow cytometry, use standard surface staining protocols with appropriately titrated antibody; consider using live/dead discrimination and appropriate isotype controls.
• Validate reactivity in relevant cell systems such as human PBMCs or cynomolgus monkey lymphocytes, as cross-reactivity is indicated.
• Depending on experimental design, fixation/permeabilization steps may be optimized if intracellular staining is required.
• For multiplex panels, check fluorophore compatibility and potential steric hindrance with other markers in the CD8 co-receptor complex.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by providing one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. We specialize in immunology targets like CD8a for T-cell profiling, offering reliable supply chains from concept to clinic. For rare or custom targets, our sourcing services bridge the gap between discovery and commercial-scale production, ensuring you get the critical reagents you need.