Anti-CD8a Monoclonal Antibody for FC - P07725

Core Product Specifications and Parameters

Biological Background: CD8a Function and Localization

• CD8a is the alpha chain of the CD8 coreceptor, a T-cell surface glycoprotein critical for immune responses against external and internal threats.
• As a coreceptor for MHC class I molecules, CD8a simultaneously binds the T-cell receptor (TCR) and MHC class I on antigen-presenting cells, recruiting the kinase LCK to initiate signaling cascades that drive T-cell activation, cytokine production, and cytotoxic responses.
• This interaction enables cytotoxic T lymphocytes (CTLs) to recognize and destroy infected or tumor cells presenting cytosolic peptide antigens.
• In natural killer (NK) cells, CD8a homodimers support target-cell conjugation and lysis, enhancing survival and serial killing capacity.
• CD8a also facilitates the differentiation of activated lymphocytes into memory CD8+ T cells, contributing to long-term adaptive immunity.
• The protein is a single-pass type I membrane glycoprotein localized to the cell membrane, featuring an extracellular immunoglobulin-like domain, a transmembrane helix, and a cytoplasmic tail for signaling.
• Post-translational modifications include disulfide bond formation, N-linked glycosylation, and palmitoylation, which influence its stability and membrane organization.
• Keywords associated with CD8a include adaptive immunity, immunity, immunoglobulin domain, and lipoprotein, reflecting its role in lymphocyte function.

Experimental Guidance and Technical Tips

• The antibody is directly conjugated to ABflo 647, making it ready-to-use for flow cytometry. Proper titration and isotype controls are recommended to optimize staining.
• The immunogen comprises the extracellular domain (aa 26-189), so the antibody is expected to recognize native cell-surface CD8a. For intracellular staining, permeabilization conditions should be validated.
• Reported cross-reactivity is with rat; performance in other species should be empirically tested if extended applications are needed.
• The recombinant fusion protein immunogen suggests high specificity; however, we advise confirming reactivity in the relevant sample system (e.g., rat splenocytes or PBMCs).

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio bridges diagnostic developers and researchers to premium-quality antibodies like this anti-rat CD8a clone, ideal for flow cytometric immune profiling. From validated antibody pairs and optimized monoclonal antibodies to ancillary reagents and rare target sourcing, we support every stage of your assay development. Let CamelBio streamline your procurement and accelerate your path from concept to clinic.