Anti-CD89 Monoclonal Antibody for Flow Cytometry - P24071

Core Product Specifications and Parameters

Biological Background: CD89 Function and Localization

• CD89, also known as Fc alpha receptor (FcalphaR) or FcalphaRI, is a type I transmembrane receptor encoded by the FCAR gene (Gene ID: 2204) in humans.
• It functions as the receptor for immunoglobulin A (IgA), binding to the Fc region of IgA and mediating downstream immune effector functions, including cytokine production.
• CD89 is expressed on the surface of myeloid cells such as monocytes, macrophages, and neutrophils, and exists in multiple isoforms (A.1, A.2, A.3) due to alternative splicing, which show differential expression between blood and mucosal myeloid cells.
• The receptor is localized predominantly at the cell membrane, but a secreted form can also be produced, which may play a regulatory role.
• CD89 contains two extracellular immunoglobulin-like domains, a transmembrane helix, and a short cytoplasmic tail, and is glycosylated.
• The theoretical molecular weight is approximately 32 kDa, consistent with the observed 32 kDa band.

Experimental Guidance and Technical Tips

• The immunogen encompasses the entire extracellular domain (aa 22-227), so the antibody may detect both membrane-bound and soluble CD89; validate in the relevant sample type.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and assay conditions to minimize background staining.
• exhibits excitation/emission maxima at ~690/720 nm, compatible with standard red lasers; ensure proper compensation when using multi-color panels.
• Consider using human monocytes or macrophage cell lines as positive controls, and verify expression based on cell type and activation status.
• The antibody is a recombinant rabbit monoclonal, offering high batch-to-batch reproducibility; however, confirm lot-to-lot consistency for diagnostic applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials, including validated antibody pairs, optimized recombinant monoclonal antibodies, and ancillary reagents, to support immunology and diagnostic research. This anti-CD89 conjugate streamlines flow cytometric detection of the IgA receptor, and we can also assist in sourcing other rare-target reagents or customized bulk preparations. Let CamelBio be your trusted partner from concept to clinic, ensuring reliable supply for your assay development and manufacturing needs.