Anti-CD86 Rabbit Polyclonal Antibody for WB, IP, IF/ICC, IF-P, ELISA - P42081

Core Product Specifications and Parameters

Biological Background: CD86 Function and Localization

• CD86 (also known as B7-2, B70, and FUN-1) is a T-lymphocyte activation antigen belonging to the immunoglobulin superfamily that functions as a costimulatory molecule essential for T-cell activation. Related references: PMID:12196291 PMID:7694363
• It serves as the primary auxiliary signal augmenting the MHC/TCR signal in naïve T-cells by binding to the CD28 receptor constitutively expressed on T-cells, playing a critical role in the early T-cell activation decision between immunity and anergy. Related references: PMID:12196291 PMID:7694363 PMID:7527824
• CD86 also acts as an inhibitor of T-cell activation by serving as a ligand for CTLA4, a decoy receptor that blocks CD28-mediated priming, thereby providing a negative regulatory checkpoint. Related references: PMID:11279501
• It is involved in B-cell regulation and modulates IgG1 production; upon CD40 engagement, activates NF-κB signaling through phospholipase C and protein kinase C.
• CD86 is a single-pass type I membrane protein localized on the cell surface of antigen-presenting cells, with a molecular weight of ~38 kDa (precursor ~33 kDa).
• It is post-translationally modified by N-linked glycosylation, disulfide bond formation, and ubiquitination, and exists in alternatively spliced isoforms.
• CD86 serves as a receptor for adenovirus subgroup B, facilitating viral entry during infection.
• The protein is classified under adaptive immunity, host-virus interaction, and immunoglobulin domain proteins.

Experimental Guidance and Technical Tips

• For Western blot (WB) analysis, consider using whole-cell lysates from human, mouse, or rat antigen-presenting cells; the antibody may detect a band around 38–50 kDa due to variable glycosylation. Validate the signal with a relevant positive control.
• When performing immunofluorescence (IF/ICC, IF-P), expect membrane localization; use a suitable fixation and permeabilization protocol to preserve the epitope.
• For ELISA applications, this polyclonal antibody can be explored as a capture or detection reagent for recombinant or native CD86; optimization of working concentrations is recommended.
• Given the polyclonal nature, lot-to-lot consistency should be verified for quantitative assays; consider pairing with a monoclonal antibody for critical applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a streamlined sourcing solution for IVD raw materials, including this anti-CD86 antibody for T-cell costimulation and immunotherapy research. As your one-stop partner, we offer validated antibody pairs, bulk ancillary reagents, and custom rare-target sourcing to support your diagnostic development from concept to clinic.