Anti-CD86 Monoclonal Antibody for WB, IF/ICC, FC, ELISA - P42081

Core Product Specifications and Parameters

Biological Background: CD86 Function and Localization

• CD86 (B7-2, LAB72) is a costimulatory molecule belonging to the immunoglobulin superfamily that plays an essential role in T-lymphocyte activation by acting as a ligand for the CD28 receptor, providing the primary auxiliary signal for naive T-cell priming. Related references: PMID:12196291, PMID:7694363

• This molecule is critical in the early decision between T-cell immunity and anergy, occurring within 24 hours of activation, thereby influencing adaptive immune responses. Related references: PMID:7527824

• CD86 also functions as an inhibitor of T-cell activation by binding CTLA4, which outcompetes CD28 and blocks CD28-mediated costimulation, acting as a decoy receptor. Related references: PMID:11279501

• In B cells, CD86 engagement with CD40 activates the NF-kappa-B signaling pathway via phospholipase C and protein kinase C, regulating IgG1 production (By similarity).

• It can serve as a receptor for adenovirus subgroup B, facilitating viral entry (microbial infection).

• CD86 is localized on the cell membrane and is expressed on the surfaces of professional antigen-presenting cells. It features an immunoglobulin V-set domain, a signal peptide, transmembrane helix, disulfide bond, and N-linked glycosylation sites. The theoretical molecular weight is approximately 37.7 kDa, consistent with the observed 38 kDa band.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 20–241, corresponding to the extracellular domain of human CD86. This antibody is expected to recognize the native surface protein, making it suitable for flow cytometry (FC) and immunofluorescence/immunocytochemistry (IF/ICC) on live cells.
• For western blot (WB), CD86 migrates at ~38 kDa. Consider using reducing conditions and validate the band in stimulated antigen-presenting cells (e.g., dendritic cells or B cells) where CD86 is upregulated.
• In ELISA applications, this antibody may be used for capture or detection of soluble CD86 or in cell-based assays. Titration and pairing with other clones are recommended.
• The antibody is specific for human CD86. Cross-reactivity with mouse or rat CD86 has not been reported; validate in the relevant sample system if needed.
• To enhance detection, activation of antigen-presenting cells with stimuli such as LPS, CD40L, or cytokines can significantly upregulate CD86 surface expression.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD manufacturers and research laboratories, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw material scouting. This CD86 antibody supports immunoassay development and research in immune checkpoint biology and T-cell costimulation. From concept to clinic, CamelBio provides the technical support and supply chain reliability to accelerate your diagnostic workflow.