Anti-CD86 (B7-2) Polyclonal Antibody for WB, IF/ICC, ELISA - P42081

Core Product Specifications and Parameters

Biological Background: CD86 Function and Localization

• CD86, also known as B7-2, is a costimulatory molecule of the immunoglobulin superfamily that provides a critical secondary signal for T-cell activation by engaging the CD28 receptor. Related references: PMID:12196291 PMID:7694363
• CD86 influences the fate of naive T-cells, contributing to the decision between immunity and anergy within 24 hours after activation. Related references: PMID:7527824
• It also serves as a negative regulator of T-cell activation by binding to CTLA4, a decoy receptor that blocks CD28-mediated priming. Related references: PMID:11279501
• CD86 is involved in the regulation of B cell function and controls IgG1 production; upon CD40 engagement, it activates the NF-kappa-B signaling pathway.
• Expressed on antigen-presenting cells, CD86 localizes to the cell membrane and has a molecular weight of approximately 38 kDa.
• CD86 acts as a host cell receptor for adenovirus subgroup B, facilitating viral entry.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 25‑133) of human CD86, which contains the ligand-binding IgV-like region—critical for CD28/CTLA4 interaction. Consider this when designing blocking experiments or epitope mapping.
• For western blotting, the predicted molecular weight is ~38 kDa. Validate the antibody in reduced and non‑reduced conditions using whole‑cell lysates from activated human lymphocytes or CD86‑expressing cell lines.
• In immunofluorescence/immunocytochemistry, expect membrane‑localized staining on antigen‑presenting cells. Include appropriate controls to confirm specificity.
• For ELISA applications, explore the antibody as a capture or detection reagent in sandwich formats, pairing it with a non‑competing clone to quantify soluble or surface‑bound CD86.

CamelBio: Your One-Stop Sourcing Bridge

As a specialized IVD raw-material supplier, CamelBio provides integrated access to validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing—all tailored to accelerate your diagnostic development. For immunology‑focused projects targeting CD86 and related checkpoints, our portfolio and sourcing network can help you secure reliable, high‑quality materials from early feasibility to commercial scale.