Anti-CD85j Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8NHL6

Core Product Specifications and Parameters

Biological Background: CD85j (LILRB1) Function and Localization

• LILRB1 (CD85j) is a leukocyte immunoglobulin-like receptor subfamily B member that functions as an inhibitory receptor for a broad range of MHC class I molecules, including HLA-A, HLA-B, HLA-C, HLA-G, and HLA-F.
  Related references: PMID:16455647 PMID:28636952

• It also recognizes the human cytomegalovirus class I MHC homolog H301/UL18, contributing to viral immune evasion.

• Ligand binding to LILRB1 triggers inhibitory signals that downregulate immune responses, including inhibition of FCER1A-mediated serotonin release and FCGR1A-mediated protein phosphorylation and calcium mobilization.
  Related references: PMID:11907092 PMID:9285411 PMID:9842885

• Engagement of LILRB1 on natural killer (NK) cells or T cells by MHC class I molecules protects target cells from lysis.

• In decidual NK cells, LILRB1 interaction with HLA-G/β2-microglobulin/peptide complexes induces secretion of growth-promoting factors, supporting maternal-fetal tolerance.
  Related references: PMID:19304799 PMID:29262349

• LILRB1 signaling reprograms B cells toward an immune suppressive phenotype.
  Related references: PMID:24453251

• LILRB1 is expressed on the cell membrane and also exists as a secreted form. It is found on B cells, monocytes, dendritic cells, decidual macrophages, and decidual NK cells.

• The protein is a transmembrane glycoprotein with immunoglobulin domains, disulfide bonds, and phosphorylation sites, classified under the immunoglobulin superfamily.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight is approximately 71 kDa; however, due to glycosylation, the observed band may migrate at a higher apparent molecular weight. Use reducing conditions and validate with relevant human cell lysates (e.g., B cells or monocytes).
• For immunofluorescence/immunocytochemistry, fixation and permeabilization protocols should be optimized to detect membrane-localized LILRB1. Consider co-staining with cell surface markers for confirmation.
• In ELISA applications, this polyclonal antibody can serve as capture or detection reagent. Pairing with a monoclonal antibody may improve specificity in sandwich ELISA setups.
• The antibody is specific to human LILRB1; cross-reactivity with other species is not expected. Validate in the relevant sample system to confirm performance.
• As a polyclonal antibody, lot-to-lot consistency should be verified for critical quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a streamlined, one-stop supply of IVD raw materials. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing, supporting every stage from concept to clinic. This anti-CD85j polyclonal antibody supports immune regulation and inhibitory signaling research, enabling the development of robust immunoassays for leukemia, immune tolerance, and viral immune evasion studies.