Anti-CD84 Monoclonal Antibody for WB and ELISA - Q9UIB8

Core Product Specifications and Parameters

Biological Background: CD84 Function and Localization

• CD84, also known as SLAM family member 5 (SLAMF5), is a self-ligand receptor that mediates cell-cell interactions and modulates the activation and differentiation of various immune cells, bridging innate and adaptive immunity.
• Homophilic CD84 interactions boost proliferative responses in activated T-cells and enhance interferon-γ (IFNG) secretion in lymphocytes, as well as induce platelet activation via a SH2D1A-dependent pathway.
• CD84 serves as a reliable marker for hematopoietic progenitor cells.
  Related references: PMID:11564780, PMID:12115647, PMID:12928397
• In mast cells, CD84 negatively regulates high-affinity IgE receptor signaling, a process independent of SH2D1A/SAP but involving FES kinase and the phosphatase SHP-1.
  Related references: PMID:22068234
• In macrophages, CD84 enhances LPS-triggered MAPK and NF-κB pathways, modulating cytokine release through its ITSM 2 motif (by similarity).
• CD84 positively regulates macroautophagy in primary dendritic cells by stabilizing IRF8 and preventing its TRIM21-mediated proteasomal degradation.
  Related references: PMID:29434592
• During the germinal center reaction, CD84 is required for prolonged T-cell:B-cell contact, optimal T follicular helper cell function, and maintaining B-cell tolerance (by similarity).
• Predominantly expressed in hematopoietic tissues and immune cells—including macrophages, B‑cells, monocytes, dendritic cells, platelets, and memory T‑cells—CD84 is a transmembrane glycoprotein located at the cell membrane, featuring disulfide bonds, phosphorylation sites, and immunoglobulin domains.

Experimental Guidance and Technical Tips

• The immunogen (aa 246–345) lies within the extracellular domain of CD84; this region may be favorable for recognizing surface‑expressed protein in applications beyond those currently validated (WB, ELISA), though further optimization would be needed.
• For Western blotting under reducing conditions, a specific band at ~39 kDa is expected; use human lymph node, spleen, or PBMC lysates as positive controls and validate with the relevant sample system.
• Cross‑reactivity with mouse and rat CD84 allows cross‑species analyses; however, we recommend pilot experiments to confirm performance in each species’ tissue or cell model.
• In ELISA setups, consider direct or sandwich formats—pair this antibody with a detection reagent targeting a separate epitope, and optimize coating concentrations to improve signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, supplying validated antibody pairs, high‑performance monoclonal antibodies like this anti‑CD84 mAb, and bulk ancillary reagents to streamline immunology research and diagnostic development. Our comprehensive portfolio meets diverse targets, including rare immune markers, ensuring reliable supply chains to accelerate your assay development from concept to clinic.